Advances in biomimetic three-dimensional (3D) melanoma models have brought new prospects of drug screening and disease modeling, since their physiological relevancy for recapitulating vivo tumor architectures is more accurate than traditional two-dimensional (2D) cell culture. Gelatin methacryloyl (GelMA) widely used as a tissue-engineered scaffold hydrogel 3D In the present study, an vitro mal...

To study prokaryotic expression and subcellular localization of α-13 giardin in Giardia lamblia trophozoites, α-13 giardin gene was amplified and cloned into prokaryotic expression vector pET-28a(+). The positive recombinant plasmid was transformed into E. coli BL21(DE3) for expression by using IPTG and autoinduction expression system (ZYM-5052). The target protein was validated by SDS-PAGE and...

The goal of this report is i) to model the occurrence and hunting yield (HY) density wild ungulates not only for widely distributed species in Europe, but also those ones which have a constrained distribution ii) compare output with observed HY. Random Forest function was used modelling species. We data available from past 30 years, HY (period 2015-2020) records collected by ENETWILD. Like prev...

6 Department of Physiology and Biophysics, Department of Microbiology and 7 Immunology, Virginia Commonwealth University, Richmond, VA 23298 USA and Kirin 8 Kyowa Foods Co. LTD, Ibaraki, Japan 300-0398. 9 10 RUNNING TITLE: Sweet and salty taste responses in P and NP Rats 11 12 Corresponding Author: Dr. Vijay Lyall 13 Associate Professor 14 Department of Physiology and Biophysics 15 Virginia Com...

The aim of this study was to evaluate the effects of sodium hyaluronate (HY), single-walled carbon nanotubes (SWCNTs) and HY-functionalized SWCNTs (HY-SWCNTs) on the behavior of primary osteoblasts, as well as to investigate the deposition of inorganic crystals on titanium surfaces coated with these biocomposites. Primary osteoblasts were obtained from the calvarial bones of male newborn Wistar...

BACKGROUND The efficacy of rotigotine has been demonstrated in studies of patients with early (i.e. not receiving levodopa) and advanced (i.e. not adequately controlled on levodopa; average 2.5 h/day in 'off' state) Parkinson's disease (PD). OBJECTIVE To further investigate the efficacy of rotigotine transdermal patch across different stages of PD symptom severity and functional disability, a...

The poor prognosis of patients suffering from malignant glioma requires further efforts. Photodynamic therapy (PDT) might be a therapeutic option to increase surgical radicality. Hypericin (HY) exhibit high phototoxicity to malignant cells and accumulates to a higher extent in glioblastoma cells as compared to neurons. Therefore, the impact of various experimental parameters on cytotoxicity, in...

OBJECTIVE To evaluate factors associated with myopic shift among primary school children. METHODS In a one-year prospective school-based study, 5052 children from ten schools were enrolled using a multi-stage random cluster approach. The baseline examination included non-cycloplegic auto-refractometry and questionnaire interview. Measurements were repeated at the follow-up. RESULTS Among 50...

In this study, 5052 Al–Mg alloy was pre-treated and friction stir processing (FSP) was then performed on the specimens to explore the deformation resistance. The experimental results indicate that the FSP specimens not only had better tensile properties but also better vibration fracture resistance. It is significant that the tensile elongation and deformation resistance tended to increase as t...

Toward minimizing bacterial colonization of surfaces, we present a one-step etching technique that renders aluminum alloys with micro- and nano-scale roughness. Such a multi-scale surface topography exhibited enhanced antibacterial effect against a wide range of pathogens. Multi-scale topography of commercially grade pure aluminum killed 97% of Escherichia coli and 28% of Staphylococcus aureus ...