Cereal Chem. 63(6):493-496 Quantitative analyses of soybean globulins were made by densitometry Proteins extracted with both water and 0.5M sodium chloride contained in a sodium dodecyl sulfate (SDS)-polyacrylamide slab gel using lysozyme glycinin (36.5%), /3-conglycinin (27.8%), y-conglycinin (6.2%), Kunitz as an internal standard protein and Coomassie Brilliant Blue as a staining trypsin inhi...

Two experiments were conducted to investigate the in vitro ability of keratinase to hydrolyze soybean glycinin and β-conglycinin and to evaluate the in vivo effects of keratinase when included in corn-soybean diets with different levels of crude protein and fed to nursery pigs. In experiment 1, a saturated keratinase solution (1 ml) was added to two blank controls of either glycinin or β-congly...

The degradation of the major seed storage globulins of the soybean (Glycine max [L.] Merrill) was examined during the first 12 days of germination and seedling growth. The appearance of glycinin and beta-conglycinin degradation products was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cotyledon extracts followed by electroblotting to nitrocellulose and immunostaining...

This study investigated the interfacial adsorption and emulsifying performance of glycated β-conglycinin (7S) with D-galactose (Gal) at various times. Results indicated that glycation increased particle sizes zeta potentials 7S by inducing subunit dissociation. Glycation destroyed tertiary structures transformed secondary from an ordered one to a disordered one, leading more flexible compared u...

Two types of cysteine proteases, low-specificity enzymes from the papain family and Asn-specific from the legumain family are generally considered to be the major endopeptidases responsible for the degradation of seed storage proteins during early seedling growth. The action of the corresponding enzymes (CPPh1 and LLP, respectively) from common bean (Phaseolus vulgaris L.) on phaseolin (the com...