The Zymomonas mobilis gene encoding acid phosphatase, phoC, has been cloned and sequenced. The gene spans 792 base pairs and encodes an Mr 28,988 polypeptide. This protein was identified as the principal acid phosphatase activity in Z. mobilis by using zymograms and was more active with magnesium ions than with zinc ions. Its promoter region was similar to the -35 "pho box" region of the Escher...

Background Zymomonasmobilis has attracted great interest in the scientific, industrial and biotechnological due to its high potential fermentation. From the viewpoint of taxonomic Z. mobilis is the only species of the genus Zymomonas, and is subdivided into three subspecies: Z. mobilis subsp. mobilis, Z. mobilis subsp. pomaceae and Z. mobilis susp. francensis. Differentiation between these thre...

Regarding some reported antimicrobial properties of tannins; Zymomonas mobilis was used to obtain ethanol from tannin-reduced carob pod extract (TR-CPE). Culture of 50 ml volume containing 7.5 g sugar at pH 5.5 and 0.03 g bacterial inoculums with shaking at 80 rpm was used. Using response surface methodology (RSM), the maximum ethanol concentration of 5.34 % w/v (higher than that reported earli...

The phoD gene encoding the membrane-bound alkaline phosphatase (ALPI) from Zymomonas mobilis CP4 was cloned and sequenced. Both the translated sequence and the properties of the recombinant enzyme were unusual. Z. mobilis ALPI was monomeric (M(r) 62,926) and hydrolysed nucleotides more effectively than sugar phosphates. The translated sequence contained a single hydrophobic segment near the N-t...

The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis consensus sequences. Extracts from E. coli cells, containing the sacA gene, display...

Isotopic and enzymic evidence indicates that Zymomonas anaerobia ferments glucose via the Entner-Doudoroff pathway. The molar growth yields with glucose (5.89) and fructose (5.0) are lower than those for the related organism Zymomonas mobilis and the observed linear growth suggests that energetically uncoupled growth occurs. A survey of enzymes of carbohydrate metabolism revealed the presence o...

this work presents a control engineering perspective of ethanol production from biomass. The bacterium Zymomonas mobilis represents an important microorganism used for this purpose but it makes the fermentation process presents high nonlinearities. Last years this continuous fermentation process has been studied and several state estimation and linear and nonlinear control tools have been devel...

C57Bl/10 male mice infected with Schistosoma mansoni were distributed into mixed, prophylactic and curative groups. A culture of Zymomonas mobilis was orally administered to mice. A 61% protection from the infection was observed in the curative group (p <0.05). Histopathological study of the livers and intestines showed similar results.