A spectrophotometric method for the determination of borohydride is described. It involves the reduction of NAD’ to a number of isomeric forms of NADH by borohydride. In the standard assay procedure herewith presented, there is a direct proportionality between the absorption at 340 nm and the amount of borohydride in solution over the range IO-100 nmoles, with an effective “molar extinction coe...

A method has been developed in which the human urinary prostaglandin metabolites are converted into stable prostanoic and prostanediotic acid homologues. The urine is reduced with sodium borohydride and the organic compounds, isolated with an Amberlite XAD-2 column, are treated with fuming hydrogen iodide, followed by treatment with zinc in methanolic hydrogen chloride, to yield dimethyl tetran...

In the light of recent reports, based on radioactive labelling studies, that substantial amounts of N-linked oligosaccharides are released from protein under the mild-alkaline borohydride degradation conditions that are usually used to release O-linked oligosaccharides, we have investigated by chemical methods the effects of alkali alone and alkaline borohydride on the carbohydrate and peptide ...

Sodium borohydride or sodium tetrahydroborate is used as a reducing agent in many organic and inorganic reactions. Therefore, the determination of sodium borohydride present in the system is of great importance. Borohydride releases hydrogen under appropriated conditions in the presence of a suitable catalyst. Since the amount of hydrogen emitted is proportional to the amount of borohydride, th...

Metal ions are present in many different biological materials, and are capable of forming strong cross-links in aqueous environments. The relative contribution of different metal-based cross-links was measured in the defensive glue produced by the terrestrial slug Arion subfuscus. This glue contains calcium, magnesium, zinc, manganese, iron and copper. These metals are essential to the integrit...

In 1941 (1) the senior author entered the field of 16-substituted steroids with the view of preparing all of the three possible 16,17-epimers of natural estriol. Since that time two of these three epimers, 16-epiestriol (isoestriol-A) (2) and 17-epiestriol (3), have become available through our efforts and those of the Swiss investigators. In 1952 the present authors made an attempt to prepare ...