We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in...

A dual-staining procedure for acid-fast bacilli was found to have poor correlation with the Ziehl-Neelsen and auramine-rhodamine staining techniques.

background: cryptosporidium is a protozoan parasite causes cryptosporidial diarrhea , which is typically a short-lasting benign infection, but can become severe and non-resolving in immunocompromised individuals. the aim of the present study was to determinate the prevalence of cryptosporidium infection in livestock and humans that were in contact with livestock in hamadan district, iran. metho...

background: tuberculosis continues to be a serious public health problem causing nearly three million deaths per year all over the world. despite major improvement in diagnosis, it is not possible to control the disease in the absence of surveillance treatment, and follow-up programs. objectives: this research was designed to study the frequency of mycobacterium tuberculosis among specimens ref...

AIM The aim of this study is evaluation of molecular assay and the standard staining method. BACKGROUND Cryptosporidium is a protozoon from coccidian subclass, which is one of the most important causes of diarrhea in children and immunocompromised individuals around the world. Diagnosis and treatment are necessary for mentioned cases. Usual diagnostic method for this parasite is fecal smear p...

Objective. To determine the Cryptosporidium spp. infection frequency by using Ziehl-Neelsen and Auramine stains on samples obtained from diarrheic calves from milking farms of the Valdivia province. To compare both diagnostic tests and to determine the geospatial distribution of the infections caused by this protozoan. Materials and methods. 221 fecal samples of diarrheic calves of 24 milking f...

Cryptosporidium parasite has gained an attention as an emerging pathogen in the last few decades. It is a zoonotic and is transmitted via the fecal-oral route; this has been implicated as one of the more important opportunistic infections in patients with Acquired immune deficiency syndrome. The results showed the presence of Cryptosporidium oocyst in fecal of sheep (11.1 and 22.2%), goats (15....