Yeast artificial chromosome (YAC) clones were arranged on the positions of restriction fragment length polymorphism (RFLP) and sequence-tagged site (STS) markers already mapped on the high-resolution genetic maps of rice chromosomes 3 and 11. From a total of 416 and 242 YAC clones selected by colony/Southern hybridization and polymerase chain reaction (PCR) analysis, 238 and 135 YAC clones were...

The recognition of mammalian genes encoded within a mouse yeast artificial chromosome (YAC) by the yeast transcription and RNA processing machinery was investigated. Transcripts from five genes known to be encoded by the YAC were all found in the total yeast RNA. Of 12 mouse introns assayed, six were correctly spliced by the yeast. However, an abnormal transcription of mouse DNA was also observ...

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that are suboptimal substrates for gene isolation. Here we report a strategy to identify gene sequences ...

Yeast artificial chromosomes (YACs) are presently the most effective means of cloning large contiguous regions of genomic DNA (1). However, performing a detailed molecular analysis of YAC clone insert DNA is constrained by inherent physical and technical limitations. Typically, an extended analysis of YAC DNA is facilitated by subcloning YAC insert DNA into lambda bacteriophage or cosmid vector...

Internet of Medical Things (IoMT) is a breakthrough technology in the transfer medical data via communication system. Wearable sensor devices collect patient and them through mobile internet, that is, IoMT. Recently, shift paradigm from manual storage to electronic health recording on fog, edge, cloud computing has been noted. These advanced technologies have facilitated services with minimum c...

To test whether yeast artificial chromosomes (YACs) can be used in the investigation of mammalian development, we analyzed the phenotypes of transgenic mice carrying two types of ,B-globin locusYAC developmental mutants: (i) mice carrying a G -> A transition at position -117 of the Ay gene, which is responsible for the Greek Ay form of hereditary persistence of fetal hemoglobin (HPFH), and (ii)...

Recent developments in genetic linkage mapping of the h uman genome have generated a large number of short tandem repeat polymorphic markers (13). Eventual integration of these markers into a-physical map is a natural and a necessary step. A large number of genomic clones have been generated for the early-onset breast and ovarian cancer (BRCA1) region at 17q21 (1-3, 5, 7, 9, 10) and are being u...

Fluorescent in situ hybridization (FISH) analysis with a panel of DNA probes for 13q13.1-q14.3 was performed on 20 cases of myeloid malignancies, of which 17 showed a del(13)(q) and three had translocations affecting 13q. By chromosome morphology, deletions consistently involved bands q14 and q21. In addition to confirming the chromosome data, FISH allowed us to delineate a commonly deleted reg...

A modified bacterial artificial chromosome (BAC) vector, pSURF-2, adapted for the selective subcloning of yeast artificial chromosome (YAC) sequences was constructed. DH10B-U, a pyrF derivative of the highly transformable E. coli strain DH10B was also constructed and used for the detection of Ura+ recombinants carrying DNA linked to YAC right arms. The vector's properties were illustrated in tw...

Tumor cells that are treated with rIL-10 or transfected with the IL-10 gene show phenotypic changes. These include low but peptide-inducible expression of MHC class I, low sensitivity to specific CTL-mediated lysis, and increased NK sensitivity. In vitro-established mouse tumor lines were screened for IL-10 expression and production, and a large proportion of plasmocytomas or T cell lymphomas w...