The vascular endothelium is a crucial interface that controls the recruitment of circulating leukocytes. Based on the luminal expression of the ephrin-B2 ligand by endothelial cells (ECs) and the expression of EphB receptors (EphBRs) by circulating monocytes, we hypothesized that EphBR-ephrinB interactions are involved in monocyte adhesion. Adhesion experiments with monocytic cells were perform...

GABA(B) is a G protein-coupled receptor composed of two subunits, GABA(B1) and GABA(B2). GABA(B1) contains an endoplasmic reticulum-retention sequence and is trafficked to the cell surface only in association with GABA(B2). To determine whether the C-terminus of GABA(B2) regulates GABA(B) trafficking, we constructed forms of GABA(B2) with various C-terminal truncations and examined their surfac...

Betanodavirus B2 belongs to a group of functionally related proteins from the sense-strand RNA virus family Nodaviridae that suppress cellular RNA interference. The B2 proteins of insect alphanodaviruses block RNA interference by binding to double-stranded RNA (dsRNA), thus preventing Dicer-mediated cleavage and the subsequent generation of short interfering RNAs. We show here that the fish bet...

Objective: To evaluate avidity of IgG anti-b2-glycoprotein I antibodies (anti-b2-GPI) in patients with antiphospholipid syndrome (APS) and systemic lupus erythematosus (SLE) in relation to thrombosis, and to demonstrate a possible affinity maturation of IgG anti-b2-GPI during the disease course. Methods: 64 sera from 32 patients (18 with primary or secondary APS, 14 with SLE without APS) and th...

A polymer derived from poly-9-bis[4-(thiophen-3,4-yloxy)biphenyl)]-9H-carbazole containing a carbazole and thiophene group, abbreviated as B2, was synthesized via the oxidation method by using FeCl3 an oxidant. Additionally, P(B2) coated onto ITO–glass surface electrochemical oxidative polymerization. UV spectra of these polymers their metal complexes (like Fe2+, Cd2+, Hg2+, Pb2+) were recorded...

The present study was designed to test the efficacy, as assessed by blastocyst production, of culturing slaughterhouse-derived bovine oocytes in Ménézo's B2 (B2) medium with or without serum and with or without buffalo rat cell (BRL) coculture. In addition, OPU-derived oocytes were cultured in B2-BRL coculture with or without serum for the first 72 h or TCM 199-BRL coculture with serum and the ...

The nucleolus is required for ribosome biogenesis. Human cells have 2 or 3 nucleoli associated with nucleolar organizer region (NOR)-bearing chromosomes. An increase in number and altered nucleolar morphology define cancer cells. However, the mechanisms that modulate nucleolar morphology and function are unclear. Here we show that in addition to localizing at the nuclear envelope, lamin B2 loca...

6,798,120 B1 9/2004 Fearing et al. 7,267,150 B2 * 9/2007 Shimizu et a1. ............. .. 156/378 7,446,423 B2 * 11/2008 Sakai et a1. ...... .. 257/783 7,678,211 B2 * 3/2010 Takasaki et a1. 156/64 7,896,047 B2 * 3/2011 Yamamoto ....... .. 156/378 7,988,803 B2 * 8/2011 Takasaki et a1. ....... .. 156/64 8,091,764 B2 * 1/2012 Suga et a1. ............. .. 228/18021 2006/0264086 A1 11/2006 Nolan 2008...