The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella typhi were compared with those of the Widal test in the serological diagnosis of typhoid fever. A veronal buffer extract yielded precipitation lines against 50 (96%) out of 52 sera collected from patients with typhoid but against none out of 62 sera obtained from control s...

Enteric fever(l) and Kala-azar (vis-ceral leishmaniasis) are endemic, in and around Patna. Kala-azar usually has an indolent course but it can present as an acute disease with fever, anorexia and vomiting(2,3). At times both the diseases are so closely similar in their presentation , that differential diagnosis is not always clear, Widal test, inspite of its limitations and fallacies is still w...

Reliability of the Widal tube agglutination test has been the subject of many controversies over the years. This study was performed to assess the effect of certain modifications on the performance of Widal test in a novel microplate assay. Sera from 37 patients (21 males; 16 females) (mean age 28 +/- 7 years) were tested in the Immunology Unit at King Khalid University Hospital, Riyadh. Among ...

Typhoid fever remains a major public health risk with more than 13 million cases occurring annually in Asia alone. Delay in diagnosis and institution of appropriate therapy especially with emergence of multi-drug resistant strains of Salmonella typhi, can be associated with significant morbidity and mortality may significantly increase the risk of adverse outcome. Traditionally, the isolation o...

BACKGROUND Enteric fever is endemic in all parts of India. The Widal agglutination test is widely used for its diagnosis. But the interpretation of Widal test depends upon the baseline titre which is prevalent amongst healthy individuals in a particular geographical area. AIM Present study was undertaken to establish the normal baseline titre in apparently healthy blood donors and also to det...

background and objective: the diagnosis of typhoid fever in children is a challenge due to the non-specific clinical picture. the current role of the widal test for diagnosis in developing countries has not been clear. materials and methods: charts were reviewed on all children ≤ 15 years of age discharged from 5 pediatric teaching hospitals in tehran from 1991 to 2004 with a diagnosis of typho...

typhoid fever is endemic in iran. isolation of s. typhi is a gold standard for diagnosis. laboratory diagnosis of s. typhi infection relies on serological tests such as the widal test. this study describes seroprevalence of to and th antibody in nonfebrile healthy and febrile with non-typhoid illness. for detection of sensitivity and specificity of widal test in typhoidal fever diagnosing widal...

Reliability of the Widal tube agglutination test has been the subject of many controversies over the years. !is study was performed to assess the e$ect of certain modifications on the performance of Widal test in a novel microplate assay. Sera from 37 patients (21 males; 16 females) (mean age 28 + 7 years) were tested in the Immunology Unit at King Khalid University Hospital, Riyadh. Among them...

We review the significance of the Widal agglutination test in the diagnosis of typhoid fever. Over 100 years since its introduction as a serologic means of detecting the presence of typhoid fever, the Widal test continues to be plagued with controversies involving the quality of the antigens used and interpretation of the result, particularly in endemic areas. Areas of concern with clinical and...

BACKGROUND There is an urgent need for an improved diagnostic assay for typhoid fever. In this current study, we compared the recently developed TPTest (Typhoid and Paratyphoid Test) with the Widal test, blood culture, and two commonly used commercially available kits, Tubex and Typhidot. METHODOLOGY For analysis, we categorized 92 Bangladeshi patients with suspected enteric fever into four g...