corophium volutator (pallas) has been recommended by the paris commission as one of the standard species for testing chemical used in offshore oil and gas exploration/production. in the present study, gut contents of corophium volutator have been analysed by electron probe microanalysis (epma) for baso4 and other heavy metal impurities after exposure tto spiked sediments following a standard as...

Glucocorticoids play a key role in mediating stress responses in vertebrates. Corticosterone (CORT) is the main glucocorticoid produced in amphibians, birds, and reptiles, and regulates several metabolic functions. The most common methods for quantifying CORT are competitive binding immunoassays: radioimmunoassay (RIA) and enzyme immunoassay (EIA). RIA has been broadly used since the 1980’s but...

Plasma membranes were isolated from the ameba Acanthamoeba castellanii by low-speed velocity centrifugation followed by equilibrium centrifugation in a sucrose gradient. The isolated membranes had a high ratio of sterol to phospholipid (0.98 moles/mole) and of phospholipid to protein (0.43 mg/mg). The plasma membranes had very low concentrations of DNA, RNA, lipid inositol, and glycerides. Glyc...

BACKGROUND The purpose of this study was to determine the safety and feasibility of whole gut washout for severe sepsis in human beings. METHODS High-volume polyethylene glycol-3500 was administered to patients with severe sepsis. Body temperature, white blood cell count, and ventilatory indexes were recorded 24 hours before and 24 hours after whole gut washout. RESULTS A significant decrea...

Currently used methodology for determining unbound drug exposure in brain combines measurement of the total drug concentration in the whole brain in vivo with estimation of brain tissue binding from one of two available in vitro methods: equilibrium dialysis of brain homogenate and the brain slice uptake method. This study of 56 compounds compares the fraction of unbound drug in brain (f(u,brai...

The current gut microbiome research relies on the fecal under assumption that represents of entire gastrointestinal (GI) tract. However, there have been growing concerns about using feces as a proxy to study microbiome. Here, we comprehensively analyzed composition and metabolites in at 14 different locations GI tracts genetically homogenous sibling pigs evaluate validity whole intestinal micro...

1. The influence of the gut microflora on protein synthesis in individual tissues and in the whole body of young chicks was investigated by the large-dose injection of [3H]phenylalanine. 2. Growth of germ-free chicks was significantly better than that of conventional controls. Wet weights of liver, spleen, duodenum, jejunum + ileum and caeca were heavier in conventional birds than in germ-free ...