Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

»Aufwachsen in (der) Gesellschaft. Eine historische Psychologie der Kindheit«. This paper develops a historical social psychology that can be used to understand young children’s social development. It compares the theoretical frameworks of three of the most important relational thinkers in the 20th century – Norbert Elias, Pierre Bourdieu, and Erich Fromm – to shed light on their attempts to in...

Objectives: The purpose of this study was to investigate if habitus, the unconscious and embodied mental structures founded early in life, can contribute to our understanding of how individuals choose a medical specialty. Methods: A qualitative approach was employed using standardized open-ended interviews. In the present research, sampling was purposive, with an aim to illuminating the study o...

We present a molecular theory to study the adsorption of different species within pH-sensitive hydrogel nanofilms. The theoretical framework allows for a molecular-level description of all the components of the system, and it explicitly accounts for the acid-base equilibrium. We concentrate on the adsorption of hexahistidine, one of the most widely used tags in bio-related systems, particularly...

This research discusses cultural reproduction in Charles Dickens' novel Great Expectations and aims to discover the process of novel. The researcher used culture (habitus field concept) theory Pierre-Felix Bourdieu. focuses on that occurred Expectations. data were analyzed using a descriptive qualitative method identify, describe analyze found coding sheet as instrument find out valid data. is ...

Case Presentation: A 66-year-old retired man presented to his physician with complaints of shortness of breath and weight gain over 1 week’s time during a recent cruise. He was not short of breath in the supine position, although his neck veins were distended. The blood pressure was 196/ 104 mm Hg; his heart rate was 92 bpm; and his cardiac rhythm was normal. He had a large habitus and was 76 i...

The polar flagellum of Vibrio alginolyticus is rotated by the sodium motor. The stator unit of the sodium motor consists of four different proteins: PomA, PomB, MotX and MotY. MotX and MotY, which are unique components of the sodium motor, form the T-ring structure attached to the LP ring in the periplasmic space. MotY has a putative peptidoglycan-binding motif in its C-terminal region and MotX...

The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SSGCID Protein Production Group at the University of Washington (UW-PPG) has developed a high-throu...