Myoblast cells, C2C12, which is an established cell line from satellite cells of skeletal muscle of C3H mouse, start to fuse and form multinucleated cells (myotubes) and begin to express creatine phosphokinase and myosin, when culture medium is changed from the growth medium to the differentiation medium. Among the 12 lectins that we tested, wheat germ agglutinin apparently suppressed the myotu...

The role of sialic acid in the interaction of sialoglycoproteins with wheat germ agglutinin was investigated by using several well characterized saccharides and sialoglycoconjugates. N-Acetylneuraminic acid and neuramin 2 + 3 lactose, in addition to N-acetyl-Dglucosamine and its fil-+ 4 oligomers were found to be inhibitors of wheat germ agglutinin-induced hemagglutination. Neuraminic acid-/?-m...

The influence of varying the amount of wheat germ agglutinin immobilized on Sepharose beads on the binding of glycoproteins to these beads was investigated. A series of wheat germ agglutinin-Sepharose gels containing between 0.10 and 10.0 mg of lectin/ml of gel was prepared, and the actual lectin content was established by acid hydrolysis of the gel followed by analysis of glycine, a major amin...

Wheat germ is reckoned valuable healthful functional food. The present investigation was performed to assess nutritional status of wheat biscuits and wheat germ fortified biscuits. Study included determination of gross chemical composition, caloric value, minerals (Mn, Ca, Fe, Cu, P, Na and K), vitamins (C, Folic acid, A, and E), and amino acid composition of wheat biscuits and 15%, 20% wheat g...

wheat germ, a highly nutritive part of wheat kernels, is separated during milling as a by-product. in this study, wheat germ was used to supplement cakes. different levels of the germ (0, 5, 10, 15 and 20%) at different particles sizes (280, 585, 890 and 1,195 µm) were added to a cake recipe. the results showed that with increasing the germ level and particle size, batter consistency and densit...

approved Redacted for Privacy Ralk S. Quatrano The activity of a highly purified plant lectin, wheat germ agglutinin (WGA), has been detected in wheat embryos as young as 25 days post-anthesis. The amount of hemagglutinating activity present in young embryos increases to a maximum level at 45 days post-anthesis. No lectin activity was found in the endosperm, roots, leaves or in a wheatembryo ti...

BACKGROUND Primary dysmenorrhea is one of the most common disorders in women and the main cause of absenteeism from work and school. OBJECTIVES Considering the anti-inflammatory properties of wheat germ, the present study examined the effects of wheat germ extract on the severity and systemic symptoms of primary dysmenorrhea. PATIENTS AND METHODS This triple-blinded clinical trial was perfo...

In an effort to clarify the mechanism by which certain plant lectins induce lymphocyte activation, we have studied amino acid (amino[14C]isobutyric acid) uptake in purified human lymphocytes exposed to mitogenic and nonmitogenic lectins. In confirmation of earlier work, mitogenic lectins (concanavalin A and two phytohemagglutinins) produced a dose-related, 2- to 10-fold increase in aminoisobuty...