The high-molecular weight (HMW) glutenin subunits of bread wheat are major determinants of end-use quality. The objective of this study was to determine the 1Dx and 1Dy subunits present in 43 synthetic hexaploid wheat (SHW) lines derived by crossing durum ‘Langdon’ to 43 Aegilops tauschii accessions. Protein samples were initially electrophoresed multiple times on SDS-PAGE gels to arrange subun...

Gluten is the main functional component of wheat, and is the main source of the viscoelastic properties in a dough. Dough properties are primarily governed by the structure of gluten, and interactions within the protein complex. While starch and water are the main components of doughs, the physical properties of a dough arise from interactions between gluten proteins, particularly the disul®de-...

To characterize and compare the protein quality of ten durum wheat genotypes grown under three cropping modalities in Algeria (subhumid Algiers, subhumid-semiarid Constantine, semiarid Sétif), profile their kernels was performed by High-Performance Liquid Chromatography (SE-HPLC RP-HPLC). The “variety” factor has a major impact, mainly on insoluble fraction (Fi), gliadin/glutenin ratio, large s...

in this study, 110 samples of bread wheat, aegilops crassa, ae. cylindrica and ae. tauschii were investigated to identify low-molecular-weight glutenin subunits. overall, the four alleles in term of the analysis of sds-page gels were detected. the ʻaʼ allele with 45% has the greatest frequency, and after that, ʻbʼ, ʻcʼ and ʻdʼ alleles were with 38.1%, 11.8% and 9.1% frequencies, respectively; h...

We have performed a detailed characterization and identification of wheat gluten proteins obtained from the Teal variety of Canadian hard red spring wheat. RP-HPLC separation of the sample into 35 fractions has reduced the spectral complexity; this was followed by MALDI mass spectrometry (MS), which showed the presence of six or fewer resolved protein components above 20 kDa in each RP-HPLC fra...

High-molecular weight (HMW (glutenin subunits are encoded by the Glu-1 loci (Glu-A1, Glu-B1 and <sp...

D hordein, a prolamin storage protein of barley endosperms, is highly homologous to the high molecular weight (HWM) glutenin subunits, which are the major determinants of bread-making quality in wheat flour. In hexaploid wheat (AABBDD), each genome contains two paralogous copies of HMW-glutenin genes that encode the x- and y-type HMW-glutenin subunits. Previously, we reported the sequence analy...

Field trials were conducted to evaluate the effect of nitrogen level on wheat protein content and composition in 16 cultivars over two years at three locations. The treatment comprised levels, 0 kg ha?1 as low 100 high nitrogen, applied top dressings 50 per ha tillering stem extension growth stages. Increased generally enhanced grain by 11.3% (11.5% vs. 12.8%). Considering determined reversed p...