The accumulation of fluoroquinolones in peripheral leukocytes is well established for several members of this family of drugs. This study documented accumulation of enrofloxacin and its active metabolite, ciprofloxacin, in canine peripheral leukocytes after oral administration at 7.5, 10, or 20 mg/kg, doses that are within the currently approved label of Baytril (Bayer Corporation Animal Health...

Pressure overload has been shown to induce mitogen activated protein kinases (MAPKs) and reactivate the atrial natriuretic factor in the heart. To test the sensitivity of these signals to pressure overload, we assayed the activity of MAPKs extracellular signal-regulated kinase, c-Jun N-terminal kinase 1, and p38 in protein lysates from the left ventricle (LV) or white blood cells (WBC) isolated...

Urinalysis is a diagnostic test that evaluates a sample of the urine for detection and assessment of a wide range of disorders such as urinary tract infection (UTI), kidney diseases and diabetes. Epithelial (or flat cells), red and white blood cells may be seen in the urine. The high amount of red blood cells (RBCs) in the urine may indicate infection, trauma, or kidney stones. Since the urine ...

The present study aimed to investigate the clinical significance of histone methylation in sepsis. A total of 43 blood samples from trauma and esophageal cancer patients with or without sepsis were collected. Immunofluorescence staining of isolated peripheral white blood cells (WBCs) was conducted. Co‑stained 293T cells served as a reference, to allow the levels of histone methylation in differ...

We investigated the mechanisms of increase in the pulmonary vascular permeability, focusing on the changes in the peripheral white blood cell (WBC) counts and the surface expression of CD18 on polymorphonuclear cells (PMNs). Anesthetized sheep with chronic lung lymph fistulas were used in this study. We infused synthetic endotoxin (LPS) at a rate of 10 ng/kg/min (i.v.) continuously for 24 hr. W...

White blood cell (WBC) segmentation, which is important for cytometry, is a challenging issue because of the morphological diversity of WBCs and the complex and uncertain background of blood smear images. This paper proposes a novel method for the nucleus and cytoplasm segmentation of WBCs for cytometry. A color adjustment step was also introduced before segmentation. Color space decomposition ...

To determine the diagnostic accuracy of Kernig's sign, Brudzinski's sign, and nuchal rigidity for meningitis, 297 adults with suspected meningitis were prospectively evaluated for the presence of these meningeal signs before lumbar puncture was done. Kernig's sign (sensitivity, 5%; likelihood ratio for a positive test result [LR(+)], 0.97), Brudzinski's sign (sensitivity, 5%; LR(+), 0.97), and ...

Key Points: • The clinical acceptance of intra-articular PRP has preceded needed scientific evidence • The relative levels of platelets, WBCs and RBCs in PRP are device and technique dependent. The optimum dose of platelets and/or WBCs as well as the necessity for activation is unknown. • A high margin of safety and anti-inflammatory effects appear consistent in human clinical studies. • Indica...

The counting and classification of blood cells allows the evaluation and diagnosis of a vast number of diseases. Through the analysis of white blood cells (WBCs) the ALL Acute Lymphocytic Leukemia, a blood cancer that can be fatal if left untreated, can be detected. Nowadays the morphological analysis of blood cells is performed manually by skilled operators. This involves numerous drawbacks, s...

Previous investigations suggest that dietary sources of polycyclic aromatic hydrocarbons (PAHs) contribute to the PAH-DNA adduct load in peripheral white blood cells (WBCs). In the current study, we measured PAH-DNA adducts by enzyme-linked immunosorbent assay in WBCs obtained from 47 California wildland (forest) firefighters at two time points (early and late) during an active forest fire seas...