نتایج جستجو برای: vp7

تعداد نتایج: 637  

2017
Shahram Jalilian Ali Teimoori Manoochehr Makvandi Milad Zandi

Background and Objectives Human rotavirus (RV) is responsible for most cases of acute gastroenteritis in infants, worldwide. Today, in vitro transcription (IVT) assay is widely used to develop efficient RNA for the biological experiments such as gene function analysis and reverse genetics. The aim of this study was to develop optimal full-length transcripts of the VP7 segment, using in vitro tr...

Journal: :Journal of virology 2008
Dianjun Cao Norma Santos Ronald W Jones Masatoshi Tatsumi Jon R Gentsch Yasutaka Hoshino

In a retrospective study of archival diarrheal stool samples collected from 1974 to 1991 at Children's Hospital National Medical Center, Washington, DC, we detected three genotype G9P[8] viruses in specimens collected in 1980, which represented the earliest human G9 viruses ever isolated. The VP7 genes of two culture-adapted 1980 G9 viruses were phylogenetically related closely to the lineage 2...

Journal: :iranian journal of virology 0
m khodabandehloo virology department, tehran university of medical sciences, tehran, iran m shamsi-shahrabadi virology department, tehran university of medical sciences, tehran, iran b bambai national research center of genetic engineering and biotechnology, tehran, iran

background and aims: in humans the group a rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...

B Bambai , M Khodabandehloo , M Shamsi-Shahrabadi ,

Background and Aims: In humans the group A rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. Introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...

Journal: :Virus research 2000
W C Wilson H C Ma E H Venter A A van Djik B S Seal J O Mecham

Previous phylogenetic analyses based on bluetongue virus (BTV) gene segment L3, which encodes the inner core protein, VP3, indicated a geographical distribution of different genotypes. The inner core protein, VP7, of BTV has been identified as a viral attachment protein for insect cell infection. Because the inner core proteins are involved with infectivity of insect cells, we hypothesized that...

Journal: :The Journal of general virology 1988
A D Hyatt B T Eaton

Core proteins VP7 and VP3 have been localized in bluetongue virus (BTV) and BTV-infected cells by immunoelectron microscopy. Gold-labelled monoclonal antibodies to VP7 gave intense labelling with purified BTV core particles and weaker labelling with both directly visualized viral particles, which require no purification, and purified virus particles. It is believed that VP7 is, in a small numbe...

2012
Van Thai Than In Hyuk Baek Hee Young Lee Jong Bum Kim Dong Hwa Shon In Sik Chung Wonyong Kim

Rotavirus and hepatitis A virus (HAV) spread by the fecal-oral route and infections are important in public health, especially in developing countries. Here, two antigenic epitopes of the HAV polyprotein, domain 2 (D2) and domain 3 (D3), were recombined with rotavirus VP7, generating D2/VP7 and D3/VP7, cloned in a baculovirus expression system, and expressed in Spodoptera frugiperda 9 (Sf9) ins...

Journal: :Journal of virology 1990
D R Maass P H Atkinson

Sucrose gradient sedimentation analysis of rotavirus SA11-infected Ma104 cells revealed the presence of oligomers of VP7, the structural glycoprotein, and NS28, the nonstructural glycoprotein. Cross-linking the proteins, either before or after sucrose gradient centrifugation, stabilizes oligomers, which can be analyzed by nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SD...

Journal: :Journal of virology 2011
Scott T Aoki Shane D Trask Barbara S Coulson Harry B Greenberg Philip R Dormitzer Stephen C Harrison

Antibodies that neutralize rotavirus infection target outer coat proteins VP4 and VP7 and inhibit viral entry. The structure of a VP7-Fab complex (S. T. Aoki, et al., Science 324:1444-1447, 2009) led us to reclassify epitopes into two binding regions at inter- and intrasubunit boundaries of the calcium-dependent trimer. It further led us to show that antibodies binding at the intersubunit bound...

Journal: :Vaccine 1999
L Wang J A Huang H S Nagesha S C Smith A Phelps I Holmes J C Martyn P J Coloe P R Reeves

The outer capsid protein of rotavirus, VP7, is a major neutralization antigen. A chimeric protein comprising Escherichia coli (E. coli) outer membrane protein A (OmpA) and part of porcine rotavirus VP7 containing all three antigenic regions (217 amino acids) was expressed in Salmonella and E. coli as an outer-membrane associated protein. Mice immunized intraperitoneally or orally, respectively,...

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