نتایج جستجو برای: vitro maturation immature oocytes antioxidant
تعداد نتایج: 573941 فیلتر نتایج به سال:
Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. ...
Background: In vitro maturation (IVM) of immature oocyte is a promising treatment option for infertile women.IVM procedures attempt to mimic the natural conditions of the follicular environment. The technique of IVM of oocytes has been successful after the first trial using culture of human oocytes in follicular fluid. The crucial part of this technique is the maturation process itself, and the...
Background: Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of cumulus cells on survival,in vitro maturation,fertilization and subsequent developmental capacity of mouse germinal vesicle stage oocytes during vitrification. Materials and...
Background and purpose: The purpose of this study was to evaluate the effect of mouse embryonic fibroblast cells conditioned medium on resumption of meiosis, in vitro maturation of immature mouse oocytes, fertilization and derived embryos development. Material and Methods: Immature oocytes denuded at the germinal vesicle (GV) stage were obtained from female NMRI mice 46-48 hrs after injecti...
Purpose: The purpose of this study was to develop an appropriate medium for in vitro maturation (IVM) of immature mouse oocytes. Materials and Methods: Germinal vesicle of female NMRI mouse oocytes (6-8 weeks old) were collected from ovaries and cultured in maturation medium MEM-a, supplemented with: 100 mlIU/ml rFSH+7.5 IU/ml hCG+5% FCS (Control group) and 2mM all- trans retinoic acid (t-RA) ...
background: repeated in vitro fertilization (ivf) failure together with ovarian hyperstimulation syndrome (ohss) is one of the distressing situations leading couples to search for alternative treatment options. for such patients with polycystic ovarian syndrome (pcos) who have experienced ovarian hyperstimulation syndrome, mild ovarian stimulation with in vitro oocyte maturation could be a prom...
Purpose: The aim of this study was evaluated the effect of β -mercaptoethanol on resumption of meiosis, In vivo maturation of immature mouse oocytes treated with DEHP and embryo development. Materials and Methods: NMRI mouse strain (4-6 weeks) were treated daily with 50,µI DEHP Group 1: 121 immature oocytes were matured in MEM-α medium contain FCS 5%, hCG 7.5 IU/ml, rFsH 100mIU/ml, Group 2: 12...
Background and purpose: Embryos produced from in vitro assisted reproductive technologies (ART) manipulations, such as parthenogenesis, are less developmentally competent than in vivo-derived embryos. This study was designed to evaluate the effect of L-carnitine treatment during IVM on maturation of immature oocytes selected by Brilliant Cresyl Blue (BCB) staining, and their subsequent developm...
Introduction: In vitro maturation (IVM) of oocytes, providing oocytes maturation out of normal conditions, is an appropriate infertility treatment system, though the clinical use of IVM is limited due to low rate of success. Accordingly, this study aimed to analyze the effect of fibroblast growth factor on in vitro maturation of immature oocytes. Methods: Immature oocytes of 20 female mice of...
The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...
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