The sensitivities of BHK-21 (C-13) and murine neuroblastoma (C-1300; clone NA) cells for the isolation of small quantities of a street strain rabies virus were compared. Suspensions of brain from mice sacrificed prior to the onset of clinical signs of rabies were used to stimulate weakly positive diagnostic specimens. The results of cell culture isolation were compared with those of the direct ...

Virus isolation was attempted on 267 out of 360 patients with wheezy bronchitis or asthma admitted to hospital during a 3-year period. Viruses were isolated on 39 occasions, the most common being respiratory syncytial virus and rhinovirus. The peak months for virus isolation were February and August. Virus isolation was significantly more common in readmissions than in first admissions (P less ...

In Cambodia, influenza A(H5N1) virus surveillance at live poultry markets (LPMs) relies on virus isolation from poultry specimens; however, virus is rarely detected by this method. We tested 502 environmental LPM samples: 90 were positive by PCR, 10 by virus isolation. Virus circulation could be better monitored by environmental sampling of LPMs.

three differentes continous cell lines (am 57. hela • vero) and primary cell culture of human embryo kidney are compared with regard to their .susceptibility for isolation of mumps v i r u s from saliva and cerebrospinal fluid. for this purfose the vero cell line appeared to be more suitable among the studied cell systems regarding the following reasons: it allows the highest pourcentage of mum...

A rapid centrifugation assay was compared with conventional tube cell culture for dengue virus isolation in both sera and autopsy samples from dengue and dengue hemorrhagic fever/dengue shock syndrome fatal cases. The rapid centrifugation assay allowed isolation of virus from 16.6% more samples than the conventional method, and it shortened the time for dengue virus detection. Finally, it allow...

conclusions after seven consecutive passages of both cell cultures, the h9n2 virus showed similar antigenicity, also no change on viral titer level and virus replication behavior in adaptation was found. the results highlighted the use of a549 cells for efficient virus isolation. results the influenza virus infectivity was not significant difference in these cells in the presence of trypsin. th...

A workshop was convened, at which seven enzyme-linked immunosorbent assays (ELISAs) were compared with virus isolation for the detection of viraemia in serial blood samples collected from six pigs at up to fourteen days after inoculation with classical swine fever virus. All ELISAs were of the double antibody sandwich type, using monoclonal and/or polyclonal antibodies to detect a variety of vi...

Case Report: Some viruses have been reported to cause respiratory and gastroenteric infections simultaneously. In this case we present isolation of human respiratory virus (RSV) type B from diarrheal sample of a 12 months' child with acute gastroenteritis. Results: The results indicated the presence of RSV subtype B genome in all three stool samples. Moreover, no sign of co-infections with oth...

A cell culture virus isolation procedure for infectious haematopoietic necrosis virus (IHNV) in the epithelioma papulosum cyprini cell line (EPC) is described. Ovarian fluid samples were collected from fish and tested for IHNV at 9 farms. The samples were inoculated in parallel on 24 h old EPC cell monolayers and in freshly trypsinized cells. The titre of the initial virus isolation and of firs...

herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) are members of the herpesviridae family. about 40% to 80% of the world populations are infected with hsv and its prevalence is high in iran. the high prevalence of this virus in the community and the ability of the virus in causing fatal diseases among immunocompromised patients, have encouraged studies to be performed on hsv and suitable cell ...