UNLABELLED A hepatitis C virus (HCV) cell culture system incorporating the JFH-1 strain and the human hepatoma cell line HuH-7 enabled the production of infectious HCV particles. Several host factors were identified as essential for HCV replication. Supplementation of these factors in nonhepatic human cell lines enabled HCV replication and particle production. Vero cells established from monkey...

objective: verotoxin is a member of shiga toxin family. this family contains ab protein toxins with an enzymatic (a) and a binding (b) compartment. cells that have receptor (gb3) are sensitive to cytotoxic effects of toxin. it has been shown that various tumor cells have gb3 receptor and are selectively sensitive to apoptotic effect of verotoxin. studies on tumor cell lines and laboratory anima...

We evaluated the expression of the Fas receptor gene in Vero cells infected with the Lederle vaccine strain of canine distemper virus using RT-PCR. Vero cells were plated, and after being grown for 24 h in MEM with 5% FBS, 80-90% confluent monolayer cultures were infected with the virus. The cells were harvested at 3, 6, 9, and 15 h post-infection. Uninfected Vero cells were used as a cont...

objective: reporter gene transfer to mammalian cells receives a great deal of attention due to its importance for molecular biology, embryology and developmental biology studies. among dna transfer technologies to eukaryotic cells, lipofection is known as the most widely used because of its easy handling procedure, low cell mortality and the natural pathway it undertakes. materials and methods:...

Background: Cumin (Cuminum cyminum L. [family Apiaceae]) seed essential oil is reported to have antiseptic activity. Until now the antiviral properties of cumin seed extracts on viruses such as herpes simplex virus-1 (HSV-1) have not been studied. The objective of this study was to investigate the in vitro effects of aqueous, methanolic and hydroalcoholic extracts of cumin seed on HSV-1 growth ...

Plaque formation in Vero cells has been induced with seven rabies serogroup viruses either cocultivated as infected BHK-21 or Aedes albopictus cells, or directly cultivated as infected mouse brain.

This work investigated the replication kinetics of the four dengue virus serotypes (DEN-1 to DEN-4), including dengue virus type 4 (DEN-4) recovered from an infectious cDNA clone, in Vero cells and in MRC-5 cells grown on Cytodex 1 microcarriers. DEN-1 strain Hawaii, DEN-2 strain NGC, DEN-3 strain H-87, and DEN-4 strain H-241 , and DEN-4 strain 814669 derived from cloned DNA, were used to infec...

Canine distemper virus (CDV) infections cause high morbidity and mortality in dogs. Changes the molecular biological characteristics of Korean CDV strain over multiple cell passages have not been reported. We investigated genetic CD1901-100 for use as an inactivated vaccine strain. Vero cells expressing dog nectin-4 gene (Vero/dNectin-4 cells) were used to adapt CD1901, which was passaged 100 t...

background: cumin ( cuminum cyminum l . [family apiaceae]) seed essential oil is reported to have antiseptic activity. until now the antiviral properties of cumin seed extracts on viruses such as herpes simplex virus-1 (hsv-1) have not been studied. the objective of this study was to investigate the in vitro effects of aqueous, methanolic and hydroalcoholic extracts of cumin seed on hsv-1 growt...