Microscopy and leukocyte esterase activity, both employed as screening techniques for urine cultures, were evaluated with respect to two distinct populations, male and female. When 424 urine specimens from males were examined, 95% of the Gram-stained smears and 91% of the leukocyte esterase tests correctly correlated with culture results, indicating significant bacteriuria. There were no signif...

PURPOSE The development of micturition in mice has been poorly studied because of the minute urine volume voided per micturition. We characterized development of the micturition pattern in young mice. MATERIALS AND METHODS Micturition in young and adult C57BL/6 strain mice, including 5 males and 4 females at age 3 weeks immediately after weaning, and 5 of each gender at ages 9 to 10 weeks, re...

Mycoplasma are the smallest free–living organisms which can grow on artificial media. Although some Mycoplasma are normal commensals, others are proven pathogens of respiratory and urogenital tracts.In this survey, 221 collected samples of 121 patients with urogenital infections (54.7%) and 100 patients with respiratory infections (45.3%) were examined. These patients were either referred to or...

We report the impact of anti-urothelial autoantibody (AUAb) on urinary bladder phenotype in BALB/c mice deficient of the FcγRIIb and PD-1. AUAb was present in serum samples from approximately half of the double-knockout (DKO) mice, as detected by immunofluorescence and immunoblots for urothelial proteins including uroplakin IIIa. The AUAb-positive DKO mice showed degeneration of urothelial plaq...

Objectives: This study was to evaluate the bacterial Infection of Urinary Tract and Glucose Level in patients presented with tertiary care hospital. Methods: Sterilized universal (glass) containers were used collecting early morning midstream urine from patients. Subjects instructed void 5mls Mid-Stream urine. Two hundred morning, mid-stream samples collected one urinary tract infection, age be...

We developed a consensus real-time PCR protocol that enables us to detect spiked bacterial 16S DNA from specimens such as water, urine, plasma, and sputum. The technique allows an exact Gram stain classification of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probes. All tested bacteria were identified correctly, and none gave a false-positive signal with the ...

Trichomonas vaginalis, a flagellate protozoan parasite commonly found in the human genitourinary tract, is transmitted primarily by sexual intercourse. Diagnosis is usually by in vitro culture method and staining with Giemsa stain. There are laboratories that use Gram stain as well. We compared the use of modified Field's (MF), Giemsa, and Gram stains on 2 axenic and xenic isolates of T. vagina...

Introduction: Purple urine bag syndrome is a rare clinical entity characterized by purple discoloration of the urinary catheter and drainage in chronically catheterized patients. Though it can be alarming to patients clinicians, considered benign but indicate an underlying tract infection. Case Series: The first case was 68-year-old woman with multiple comorbidities who admitted orthopedic ward...