A qualitative method, involving supported liquid–liquid extraction (SLE) and ultra high pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS–MS), was developed for the rapid tentative identification of various drugs of abuse in urine. In this study, 28 drugs and metabolites were covered by the screening procedure. Before analysis, urine samples were extracted by SLE and ...

A fast method was developed by ultra high performance liquid chromatography (UHPLC) for simultaneous determination of polyphyllin I and polyphyllin II. Chemometric analyses including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) based on UHPLC chromatography were used to evaluate 38 batches from six species of Paris. Variable importance of projectio...

An unknown impurity formed during stability sample analysis by a gradient reversed phase ultra-high pressure liquid chromatography (UHPLC) of varenicline tablets at 0.2% level. A simple isocratic preparative method was developed to isolate the unknown impurity with 20 min run time. This unknown impurity was identified and characterized by using spectroscopic techniques. Based on the spectral da...

Counterfeiting in the pharmaceutical industry has become a serious and underreported problem, particularly in developing countries. This Application Note shows the analysis of antidiabetic drugs of the sulfonylurea class using the Agilent 1220 Infinity Gradient LC system with diode array detector (DAD). Both HPLC and UHPLC methods revealed excellent precision, accuracy and linearity as well as ...

Environmental contaminants can affect human and animal health are thus an issue of concern. Anthony Provatas is a Research Professor the Organics Laboratory Manager at University Connecticut in Center for Sciences Engineering (CESE) Institute Environment; he also Program Chair Board Member Eastern Analytical Symposium (EAS). His research has been focused on effects health. We recently spoke to ...

BACKGROUND Chinese medicinal herbs may use more than one species of Curcumae Radix (Yujin) is the tuberous roots of Curcumae wenyujin, C. kwangsiensis, C. phaeocaulis and C. longa. This study aimed to characterize the chemical profiles of these different species of Curcumae Radix, and develop a method for rapid discrimination of these species by ultra-high performance liquid chromatography-quad...

To determine the monoamines dopamine (DA), noradrenaline (NAD) and serotonin (5-HT) in microdialysis samples, ion-pair liquid chromatography (LC) with electrochemical detection (ECD) is typically applied. Because of the low concentrations in the extracellular space (pM range), further dilution in the microdialysis samples, the complexity of the samples and the required temporal resolution, the ...

BACKGROUND Murraya exotica L., a member of family Rutaceae is rich in biologically active secondary metabolites and also known for its ethnobotanical importance. With this in mind, the plant was chosen and leaves were extracted sequentially to obtain ethanol, hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. The extract/fractions were evaluated for total phenolic and flavonoid...

Two analytical platforms, gas chromatography (GC) coupled to quadrupole-time-of-flight (QTOF) mass spectrometry (MS) and reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled to diode array (DAD) and QTOF detection, were applied in order to study the alkaloid profile of Fumaria capreolata. The use of these mass analyzers enabled tentatively identifying the alkaloids by matc...

Traditional Chinese medicine (TCM) has been used for the treatment of many complex diseases. However, the bioactive components are always undefined. In this study, a bioactivity-based method was developed and validated to screen lipase inhibitors and evaluate the effects of processing on the lipase inhibitory activity of TCM by ultrahigh performance liquid chromatography coupled with quadrupole...