Vaccine virus, suspended in a mixture of serum and Tyrode's solution and separated by collodion membranes from a suspension of living kidney cells in serum and Tyrode's solution, remained active at 37 degrees C. for a longer period of time than did vaccine virus incubated only in a mixture of serum and Tyrode solution.

Rabies virus has been propagated in serum-Tyrode solution containing either embryo mouse brain or embryo chick brain. The culture virus reached a titre of 3 x 10(-5) cc. after 4 days' incubation at 37 degrees C., and survived at least 2 months at 5 degrees C. in the liquid or dry state.

For investigation of the properties of smooth muscle contraction hypertonically added 40 mm K medium has been used as a routine in this laboratory. Others have used isotonic high K/Na deficient medium for the same purpose. The distinct difference xisting between the mechanical response of guinea pig taenia coli in hyper-40 K medium and that in iso 152 K medium has been reported earlier. The res...

The effects of changing the intracellular Ca concentration ([Ca]i) on the calcium current (iCa) were studied in isolated single ventricular cells of the guinea-pig. [Ca]i was varied by an intracellular perfusion technique using a suction pipette. iCa measured from internally perfused cells at a pCa lower than 9.0 was dependent on the extracellular Ca concentration ([Ca]o). Increasing [Ca]o from...

background: control of the medium osmolarity and temperature during long or short time sperm manipulation is essential. objective: the objectives of the present study were to find the effects of different osmolarities of modified tyrode's solution and milk on the bull sperm during incubation at above zero temperatures. materials and methods: semen samples were collected twice from five najdi bu...

It is known that digoxin, which is a liposoluble cardiac glycoside, is well absorbed from intestine. In the present study, the absorption rates of digoxin from rat duodenum and the proximal and terminal parts of small intestine were determined in vitro. The isolated everted duodenum and intestinal sacs were put into oxygenated Tyrode solution at 37 degrees C. The Tyrode solution on the outer, m...

In this data article, intracellular Ca(2+) concentration ([Ca(2+)]i) was measured in isolated ventricular Wild Type (WT) and mdx cardiomyocytes in two different conditions: at rest and during the application of an axial stretch. Using a carbon microfibers technique, axial stretch was applied to mimic effects of physiological conditions of ventricular filling. A study of cation entry with the sa...