The Escherichia coli tRNA(Tyr) gene carried by the varphi80psu(3) + transducing phage was transcribed in vitro by DNA-dependent RNA polymerase. The enzymatically synthesized tRNA(Tyr)-like polynucleotide chains were detected by competition with purified E. coli(32)P-tRNA(Tyr) for specific hybridization sites on varphi80psu(3) + DNA. Analysis by sucrose gradient centrifugation showed the tRNA(Ty...

We report a patient with an undetermined leucodystrophy associated with type 1A oculocutaneous albinism (OCA). Type 1 OCA results from recessive mutations in the tyrosinase gene (TYR) located in 11q14.3. The patient was found by FISH to carry a deletion of at least the first exon of the TYR gene on one chromosome and a (TG) deletion at codon 244/245 on the second chromosome. The existence of th...

Oculo-cutaneous albinism type 1 (OCA1) is characterized by congenital hypopigmentation and is due to mutations in the TYROSINASE gene (TYR). In this study, we have characterized the morpho-functional consequences of the lack of tyrosinase activity in the spontaneous null mouse model of OCA1 (Tyr(c-2j)). Here, we show that adult Tyr(c-2j) mice have several retinal functional anomalies associated...

Reversible protein phosphorylation is a widespread post-translational modification fundamental for signaling across all domains of life. Tyrosine (Tyr) has recently emerged as being important plant receptor kinase (RK)-mediated signaling, particularly during immunity. How Tyr regulates RK function however largely unknown. Notably, the expansion phosphatase and SH2 domain-containing families, wh...

Tyrosinase is a key enzyme in melanin biosynthesis. Mutations in the gene encoding tyrosinase (Tyr) cause oculocutaneous albinism (OCA1) in humans. Alleles of the Tyr gene have been useful in studying pigment biology and coat color formation. Over 100 different Tyr alleles have been reported in mice, of which ≈24% are spontaneous mutations, ≈60% are radiation-induced, and the remaining alleles ...

A unique poly(amido amine) (PAMAM)/polymer dots (Pdots)/NiO heterostructure is developed for photocathodic detection of tyrosinase (TYR), which applicable to TYR in actual human serum samples.

Science the biological activities of chiral enantiomers are often different or even opposite, their recognition is great significance. A new assembly structure named TCPP-Zn-(S)-BINOL was obtained based on interaction between binaphthol (BINOL) and porphyrin-based MOF formed by Meso-Tetra(4-carboxyphenyl)porphine (TCPP) Zn2+, a sensor designed relying TCPP-Zn-(S)-BINOL. The platform using as re...

Expression of 5 yeast mitochondrial tRNA genes (Ala, Ile, Tyr, Asn and Metm), localized upstream from the oxil gene has been analyzed by in vitro capping using guanylyltransferase, northern hybridization and S1 nuclease mapping in the wild type and a rho-strain. The 5 tRNA sequences belong to the same transcriptional unit which is initiated 133 bp upstream from the tRNA(Ala) gene at a promoter ...

a graphene nanosheets (gns) film coated glassy carbon electrode (gce) was fabricated for sensitive determination of tyrosine (tyr). the gns-based sensor was characterized by scanning electron microscope and electrochemical impedance spectroscopy. the voltammetric techniques were employed to study electro-oxidation of tyr. the results revealed that the modified electrode showed an electrocatalyt...

Three allatostatins have been isolated from the mosquito Aedes aegypti. These peptides have the following structures: Ser-Pro-Lys-Tyr-Asn-Phc-Gly-Leu-amide, Leu-Pro-His-Tyr-Asn-Phe-Gly-Leu-amide, and Arg-Val-Tyr-Asp-Phe-Gly-Leu-amide. A cDNA encoding these peptides was isolated from an abdominal ganglia cDNA library and sequenced. It was found to encode two additional allatostatins: Ala-Ser-Ala...