Bound biotin-saturated cells were incubated in the presence of biotin and glucose (37 C, pH 7.5) with or without oleic acid, Tween 20, 40, 60, and 80, Aerosol OT, sodium dodecyl sulfate (SDS), cetyltrimethylammonium bromide, Triton X-100, Non-Ion-Ox, and Haemo-Sol. With low concentrations (up to 5 mug/ml) and short reaction times (up to 10 min), oleic acid stimulated free biotin accumulation. I...

The effects of Tween-20 and Zn(II) on ethylbenzene removal were evaluated using two biotrickling filters (BTFs), BTF1 and BTF2. Only BTF1 was fed with Tween-20 and Zn(II). Results show that ethylbenzene removal decreased from 94% to 69% for BTF1 and from 74% to 54% for BTF2 with increased organic loading from 64.8 to 189.0 g ethylbenzene/(m³·hr) at EBRT of 40 sec. The effect of EBRT (60-15 sec)...

Under appropriate conditions, oleic acid replaces biotin for several lactic acid bacteria.‘, z This growth-promoting action of oleic acid is not nullified by excess avidin for Streptococcus jaecalis R2 or for several other organisms (Table I). Egg white did, however, block utilization of oleic acid by Lactobacillus arabinosus (Table I). This result is obtained whether oleic acid is added alone,...

Dalam pembuatan kosmetik salah satu proses yang paling penting adalah pengemulsian air dan minyak. Agar kedua bahan ini dapat menyatu di butuhkan emulsifier yaitu Tween 80. Penelitian bertujuan untuk mengetahui pengaruh tween 80 waktu pengadukan terhadap karakteristik meliputi nilai viskositas, bobot jenis, pH homogenitas berdasarkan SNI-163499-1996. Proses pencampuran dilakukan dengan memvaria...

Diffuse groundwater pollution from agricultural land use may impact the microbial groundwater community, which was investigated as Community-Level Physiological Profiles (CLPP) using EcoPlate™. Water was sampled from seven piezometers and a spring in a small agricultural catchment with diffuse herbicide and nitrate pollution. Based on the Shannon-Wiener and Simpson's diversity indices the diver...

Studies of alpha-amylase production by Bacillus subtilis (CM3) isolated earlier from cow dung microflora, were carried out. The optimum temperature, pH and incubation period for amylase production were 50-70 degrees C, 5.0-9.0 and 36 h, respectively. Enzyme secretion was very similar in the presence of any of the carbon sources tested (soluble starch, potato starch, cassava starch, wheat flour,...

background: tween as a substitute for diethyl ether in the formalin-ether sedimentation technique was evaluated for para­site detection. methods: fresh fecal material free of parasites with 10% formalin to prepare standardized specimen was thoroughly pooled. this specimen was divided into 5 equal portions; one was without infection, and each of the others was individually seeded with entamoeba ...