introduction: in this study, the effect of para-nonylphenol as an environmental pollutant on viability, morphology and proliferation of bone marrow mesenchymal stem cells was investigated. methods: bone marrow mesenchymal stem cells of rat were treated with the 0.5, 1, 2.5, 3.5 and 5 μm of paranonylphenol for a period of 21 days, then the viability of the cells were estimated using trypan blue ...

Background: Globozoospermia is an uncommon disorder of sperm morphology associated with severe male infertility. Patients who contain both normal and round-headed sperm are called partial globozoospermic. It is possible that round-headed sperm may have an abnormal chromatin structure and /or DNA strand breaks. Moreover, sperm DNA fragmentation and protamine deficiency is shown to be associated ...

introduction: repetitive cortical spreading depression (csd) can lead to cell death in immature brain tissue. caspases are involved in neuronal cell death in several csd-related neurological disorders. yet, whether repetitive csd itself can induce caspase activation in adult or juvenile tissue remains unknown. inducing repetitive csd in somatosensory cortices of juvenile and adult rats in vivo,...

We evaluated the effect of a basic fibroblast growth factor (bFGF) and saporin conjugate (bFGF-SAP) on proliferation, migration and tubule formation in bovine choriocapillary endothelial cells (BCECs). Cell proliferation and MTS assays were done with 0.01, 0.1, 1, 10, and 100 nM bFGF-SAP, and an equimolar concentration of bFGF and saporin. TUNEL assay was performed to confirm apoptosis. Cells w...

It is well known that transit through the epididymis involves an increase in the compaction of sperm chromatin, which acquires fully condensed status at the caput epididymidis. The purpose of this study was to compare the terminal deoxyribonucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay, the comet assay, the sperm chromatin structure assay (SCSA) and the sperm chromatin d...

One hallmark of apoptosis is the degradation of chromosomal DNA. We cloned the Caenorhabditis elegans gene nuc-1, which is involved in the degradation of the DNA of apoptotic cells, and found that nuc-1 encodes a homolog of mammalian DNase II. We used the TUNEL technique to assay DNA degradation in nuc-1 and other mutants defective in programmed cell death and discovered that TUNEL labels apopt...

OBJECTIVES To standardize the TUNEL assay by establishing inter- and intraobserver variability, interassay variability, cutoff values, sensitivity and specificity of the assay, and studying the distribution of the DNA damage in a population of infertile men referred to a clinical andrology laboratory. METHODS Seminal ejaculates from 25 healthy male volunteers (controls) and 194 infertile men ...

Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two Japanese Black bulls (A, B) several times o...