Objective(s): In this study a series of novel colchicine-like β-acetamidoketones was designed and synthesized as potential tubulin inhibitorsMaterials and Methods: The cytotoxicity of the novel synthesized β-acetamidoketones was assessed against two cancerous cell lines including MCF-7 (human breast cancer cells) and A549 (adenocarcinomi...

vinblastine is an important anticancer agent known to diminish microtubule assembly. ab initio calculations are applied to examine the structural properties and different energies of vinblastine-tubulin complex in different dielectric constants and temperatures. the aims of this work are discovery the best optimized structure and thermodynamic properties of vinblastine-tubulin complex and compa...

conclusions it was shown that partial β-tubulin sequence, as a reliable genetic target, supported specific morphological criteria for identification of the penicillium species. like other assessments throughout the world, p. chrysogenum remains the most frequent environmental penicillium species in mazandaran and tehran provinces. results sequence analysis of bt2 and morphological criteria of 2...

The interaction of tubulin-microtubule poison complexes with anti-tubulin antisera has been investigated using radioimmunoassay. The binding of the major antiserum used in this study to tubulin does not interfere with the binding of colchicine to the tubulin or affect the decay of the colchicine-binding activity of the tubulin. Conversely, if colchicine is incubated with the tubulin, forming tu...

Abstract Background and Aims Minimal change disease (MCD) is a podocytopathy more commonly seen in children, but it also accounts for 10%–25% of adult NS. Patients with MCD manifest abundant albuminuria, which related to the damage glomerular filtration barrier. Podocytes are major components barrier play crucial role maintaining integrity Alterations podocyte actin critically affect function. ...

Background: Anti-tubulin agents are widely used in the treatment of lymphoma and included important chemotherapy schemes (R-CHOP, ABVD, BEACOP) as well innovative therapeutic approaches utilizing antibody-drug conjugates (ADCs) such brentuximab vedotin polatuzumab vedotin. Recently, we have devoted our efforts to investigation a large family [1,2]oxazole-based derivatives that showed anti-proli...

Tubulin is the major protein component of brain tissue. It normally undergoes a cycle of tyrosination-detyrosination on the carboxy terminus of its alpha-subunit and this results in subpopulations of tyrosinated tubulin and detyrosinated tubulin. Brain tubulin preparations also contain a third major tubulin subpopulation, composed of a non-tyrosinatable variant of tubulin that lacks a carboxy-t...

Microtubule dynamics and polarity stem from the polymerization of αβ-tubulin heterodimers. Five conserved tubulin cofactors/chaperones and the Arl2 GTPase regulate α- and β-tubulin assembly into heterodimers and maintain the soluble tubulin pool in the cytoplasm, but their physical mechanisms are unknown. Here, we reconstitute a core tubulin chaperone consisting of tubulin cofactors TBCD, TBCE,...

We describe the interaction of pure brain tubulin with purified membranes specialized in different cell functions, i.e., plasma membranes and mitochondrial membranes from liver and secretory granule membranes from adrenal medulla. We studied the tubulin-binding activity of cellular membranes using a radiolabeled ligand-receptor assay and an antibody retention assay. The tubulin-membrane interac...

The assembly of the axoneme, the structural scaffold of cilia and flagella, requires translocation of a vast quantity of tubulin into the growing cilium, but the mechanisms that regulate the targeting, quantity, and timing of tubulin transport are largely unknown. In Chlamydomonas, GFP-tagged α-tubulin enters cilia as an intraflagellar transport (IFT) cargo and by diffusion. IFT-based transport...