(Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)). Leguminous seeds (Fabaceae) have a high content of inhibitors of which serine protease inhibitors are the most widely studied. However, there are only a few studies related to the investigation of these proteins in tree species belonging to the Amazon flora. The protein content presented in seeds of four Amazonian Legumi...

Human mesotrypsin is an isoform of trypsin that displays unusual resistance to polypeptide trypsin inhibitors and has been observed to cleave several such inhibitors as substrates. Whereas substitution of arginine for the highly conserved glycine 193 in the trypsin active site has been implicated as a critical factor in the inhibitor resistance of mesotrypsin, how this substitution leads to acc...

We have recently reported (2) the discovery and crystallization of a stoichiometric trypsin inhibitor from bovine colostrum. Several properties of this inhibitor indicated a close similarity to the two trypsin inhibitors from bovine pancreas, the first crystallized by Kunitz and Northrop (3) and the second crystallized by Kazal, Spicer, and Brahinsky (4). It was of interest to examine the compa...

The complex formed between anionic salmon trypsin (ST) and bovine pancreatic trypsin inhibitor (BPTI) has been crystallised, and the X-ray structure has been solved using the molecular replacement method. The crystals are hexagonal and belong to space group P6(1)22 with lattice parameters of a = b = 83.12 A and c = 222.15 A. Data have been collected to 2.1 A and the structure has been refined t...

MacFarlane and Biggs (5) recently reviewed the literature dealingwith the plasminogen-plasmin, an active proteolytic enzyme and an antiprotease which are present in plasmas in health and dis ease. Satisfactory quantitative data for the plas minogen-plasmin relationship and for the spon taneously active proteolytic enzyme in disease are limited. Although the antiprotease has been studied rather ...

The rotavirus capsid is made up of three concentric protein layers. The outer layer, consisting of VP7 and VP4, is lost during virus entry into the host cell. Rotavirus field isolates can be adapted to high-titre growth in tissue culture by treatment with trypsin and by supplementing the culture medium with trypsin, which cleaves VP4 into two fragments, VP8* and VP5*. It is known that protease ...

Acute pancreatitis is a life-threatening inflammatory disease characterized by abdominal pain of unknown etiology. Trypsin, a key mediator of pancreatitis, causes inflammation and pain by activating protease-activated receptor 2 (PAR(2)), but the isoforms of trypsin that cause pancreatitis and pancreatic pain are unknown. We hypothesized that human trypsin IV and rat P23, which activate PAR(2) ...

The plasma esterase inhibitors alpha2-macroglobulin, alpha1-antitrypsin, C1-inhibitor, antithrombin-heparin cofactor, and, as previously described, soybean trypsin inhibitor (Kunitz) and diisopropylphosphorofluoridate (9) enhance the response of guinea pig macrophages to migration inhibitory factor. To obtain this effect, macrophages are incubated with inhibitors prior to assay. The data sugges...

This study describes the relationship between the two major trypsin inhibitors (TI) in soybean, i.e., the Kunitz (KTI) and Bowman-Birk (BBI) trypsin inhibitors, as well as between them and the corresponding trypsin inhibitor activity (TIA). Twelve investigated soybean genotypes showed significant differences in TI levels and TIA. A very strong positive correlation was found between the levels o...

For expressing trypsin inhibitor activity (TIA), units inhibited (TUI), inhibited, and inhibitors have been used. Although the last two are preferred, their calculations in current practices require refinement. With proposed AOCS method Ba 12a-2020, four experiments were conducted, using preparations having specific of 11,625, 12,602, 13,728, 14,926 N?-benzoyl-L-arginine ethyl ester (BAEE) unit...