PURPOSE Test-retest variability (TRV) limits our ability to detect clinically significant changes in visual acuity (VA). We wanted to compare the effect of scoring and termination rules on TRV for logMAR charts, employing either conventional or pseudo high-pass (Vanishing Optotype) letters. METHODS VA measurements and TRV were compared in 50 uncorrected normal observers (17 male, mean age 42....

Cell motility is an essential element of tumor dissemination, allowing organ infiltration by cancer cells. Using mouse LB lymphoma cells transfected with standard CD44 (CD44s) cDNA (LB-TRs cells) or with the alternatively spliced CD44 variant CD44v4-v10 (CD44v) cDNA (LB-TRv cells), we explored their CD44-dependent cell migration. LB-TRv cells, but not LB-TRs or parental LB cells, bound soluble ...

Aim: To study learning effect (LE) and test retest variability (TRV) in healthy subjects and patients with primary open angle glaucoma (POAG) using Rarebit perimetry (RBP). To determine normative ranges of RBP. Methods: 61 eyes of 35 subjects underwent visual field testing with standard automated perimetry (SAP) and RBP. TRV and LE were assessed in repeated examinations conducted in 3 different...

Virus-induced gene silencing (VIGS) is a widely used, powerful technique for reverse genetics. VIGS vectors derived from the Tobacco rattle virus (TRV) are among the most popular for VIGS. We have developed a TRV RNA2 vector that allows the insertion of gene silencing fragments by ligation-independent cloning (LIC). This new vector has several advantages over previous vectors, particularly for ...

The viruses infecting tulips have a big influence on the yield and the quality of bulbs and forced flowers. Commercial bulb production is based on clonal propagation, which leads to the accumulation of viruses. Among 22 viruses occurring in tulips, the most common and the most dangerous are Tulip breaking virus, TBV; Tobacco necrosis virus, TNV; Lily symptomless virus, LSV; Cucumber mosaic viru...

Transferrin (Tf) receptor-variant Chinese hamster ovary cells have been isolated by selection for resistance to two Tf-toxin conjugates. The hybrid toxins contain Tf covalently linked to ricin A chain or a genetically engineered diphtheria toxin fragment. The Tf-receptor-variant (TRV) cells do not have detectable cell-surface Tf receptor; they do not bind fluorescein-Tf or 125I-Tf. TRV cells ar...

A microinjection technique was devised for inoculation of single Nicotiana clevelandii leaf trichome cells with virus particles. By removing inoculated trichomes at various times after microinjection, it was shown that at least 4 hr were required for tobacco rattle virus (TRV; tobravirus group) to move out of primarily inoculated cells. Effects of the early stages of TRV infection on plasmodesm...

OBJECTIVE To determine the prevalence of pulmonary hypertension (PH) in patients with cystic fibrosis (CF), to compare clinical characteristics, radiographic scores, pulmonary function, and echocardiographic parameters in patients with and without PH, and to correlate echocardiographic findings with clinical characteristics, radiographic scores, and pulmonary function. METHODS This was a pros...

Citation: Shah N, Dakin SC, Whitaker HL, Anderson RS. Effect of scoring and termination rules on test–retest variability of a novel high-pass letter acuity chart. Invest Ophthalmol Vis Sci. 2014;55:1386–1392. DOI: 10.1167/iovs.13-13340 PURPOSE. Test–retest variability (TRV) limits our ability to detect clinically significant changes in visual acuity (VA). We wanted to compare the effect of scor...

Electrical power systems are exposed to transient disturbances that change the voltage and current signals of network, which can interrupt damage equipment. In high-frequency phenomena, it is essential study recovery (TRV) ensure electrical insulation limits circuit breakers not violated, thus leading a safe reliable operation. Adequate models crucial achieving satisfactory results in studies, ...