leaf and root explants from 10 to 20 day– old seedlings of plantago lanceolata, were cultured on ms, msh, nt and b5 basal media with various concentrations of different plant growth regulators. the best callus induction was obtained with 0.4 mgl-1 2,4-d in msh i with 400 callus index. callus growth was significantly stimulated (p=0.05)with 0.8 mgl-1 2,4-d and 0.1 mgl-1 kin in msh ii, with 7...

Background: Trigonella foenum-graecum L. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline. Objective: In this study, hairy roots culture as a novel method for trigonelline production was evaluated. Methods: For optimizing the hairy roots culture of Trigonella foenum-graecum, three strains of Agrobacterium rhyzogenes (ATCC15834, MSU440 and K...

Polysaccharide mucilage derived from the seeds of Plantago major L. (family Plantaginaceae) was investigated for use in matrix formulations containing propranolol hydrochloride. HPMC K4M and tragacanth were used as standards for comparison. The hardness, tensile strength, and friability of tablets increased as the concentration of mucilage increased, indicating good compactibility of mucilage p...

Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall. However, it is not yet known how mannans function in Arabidopsis (Arabidopsis thaliana) seed mucilage. In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed co...

BACKGROUND Trigonella foenum-graecum (L.) (Fabaceae, Fenugreek) is an important ingredient of Ayurvedic and other marketed herbal formulations. Fenugreek seeds are employed in many traditional systems as an antibacterial and antidiabetic agent, gastric stimulant and galactogogue. Trigonelline, a major phytoconstituent found in fenugreek seeds, shows estrogenic, anti-diabetic and anti-invasive a...

background: trigonella foenum-graecum l. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline. objective: in this study, hairy roots culture as a novel method for trigonelline production was evaluated. methods: for optimizing the hairy roots culture of trigonella foenum-graecum, three strains of agrobacterium rhyzogenes (atcc15834, msu440 and k599) v...

fenugreek (trigonella foenum-graecum l.) has a long and respected history of medicinal uses in middle east and persian medicine. the hypocholesterolaemic and hypoglycaemic effects of fenugreek were attributed to its major steroidal sapogenin, diosgenin and its major alkaloid, trigonelline. the knowledge of diosgenin and trigonelline biosynthesis is derived from studies of cholesterol and nicoti...