Use of phylogenetic species concepts based on rDNA internal transcribe spacer (ITS) regions have improved the taxonomy of dermatophyte species; however, confirmation and refinement using other genes are needed. Since the calmodulin gene has not been systematically used in dermatophyte taxonomy, we evaluated its intra- and interspecies sequence variation as well as its application in identificat...

A rapid two-step DNA extraction method and a multiplex PCR for the detection of dermatophytes in general and Trichophyton rubrum specifically were developed and evaluated with DNA extracted from pure cultures and from clinically diseased nails. DNA from the following dermatophytes was used: Epidermophyton floccosum, Microsporum audouinii, Microsporum canis, Microsporum gypseum, Microsporum nanu...

Studies of three monoconidial subcultures of each of 10 isolates of Trichophyton soudanense on seven trichophyton agars revealed variations in growth among the subcultures of each isolate and among the isolates themselves on six agars. In contrast, greater consistency and generally good to excellent growth were noted with all isolates on trichophyton agar 1 (basal medium). These results are con...

BACKGROUND The high degree of phenotypic similarity among Trichophyton species makes their identification difficult. OBJECTIVES The current study aims to establish the use of rolling circle amplification (RCA) based on internal transcribed spacer ribosomal DNA (ITS rDNA) as a powerful, simple, and rapid procedure for distinguishing closely related organisms, and specifically to identify Trich...

Dermatophytosis and superficial mycosis are a major global public health problem that affects 20-25% of the world's population. The increase in fungal resistance to the commercially available antifungal agents, in conjunction with the limited spectrum of action of such drugs, emphasises the need to develop new antifungal agents. Natural products are attractive prototypes for antifungal agents d...

An isolate of Arthroderma simii was successfully mated with a tester strain of A. vanbreuseghemii cultured on the plate of simple agar with some hair on it at 27 degrees C. Confirmation of sexual reproduction was made by the detection of hybrids of two parental genotypes. The implications of this result are discussed from the viewpoint of a reevaluation of the species boundaries of dermatophytes.

background and objectives: cattle dermatophytosis (syn. cattle ringworm), an important skin infection, has received major consideration not only for economical losses in the animal breeding industry but also in regards to its zoonotic transmission to humans. for effective control measures, it is important to determine the disease prevalence in cattle herds. materials and methods: to determine r...

Tinea refers to superficial infection with one of three fungal genera-Microsporum, Epidermophyton, or Trichophyton-that are collectively known as dermatophytes. These infections are among the most common diseases worldwide and cause chronic morbidity. They are usually diagnosed by direct microscopy and fungal culture, which are burdensome to perform in the clinical setting. To supplement conven...

Various dermatophyte isolation media were quantitatively appraised by colony counts for their ability to support growth when suspensions of trypsin-treated infected-skin scales were used as inocula. Evaluation included guinea pig skin scales infected with a granular Trichophyton mentagrophyton mentagrophytes, and one trial was conducted using human skin scales infected with Trichophyton rubrum....