Triacylglycerols have the potential to supply significant fatty acids for ATP production by the myocardium, yet at present the exact contribution of triglyceridederived fatty acid to the energy economy of the myocardium is unresolved. Chylomicrons and very-low-density lipoproteins both add to energy production but the degree of provision may be related to particle size of individual lipoprotein...

A new micro-procedure for the quick regiospecific analysis of triacylglycerols (TAG) with a 1,3-specific lipase, Lipozyme IM 20 (produced from strains of Muccor miehei) is described. After dissolution of triacylglycerols in butan-1-ol or butan-2-ol 250 mg of Lipozyme IM 20 were added and the whole mixture was agitated. This operation was repeated 5-10 times and the esterified fatty acids in the...

This work was undertaken to determine the effect of diets enriched with olive oil or high oleic sunflower oil on very low density lipoprotein (VLDL) triacylglycerol composition of healthy human subjects. Both oils contain a similar proportion of monounsaturated fatty acids (MUFA) but differ in their triacylglycerol composition. All 22 human subjects initially consumed a low fat, high carbohydra...

A low lipase activity from a crude extract of Arabidopsis seedlings was assayed using three sensitive methods (radiolabelled triacylglycerols, commercial resorufin ester and triacylglycerols containing the naturally fluorescent parinaric acid as substrates). The specific activity of the extract was found to be similar using the three methods. However, the plant lipase activity measured using th...

All eukaryotic organisms and even a few prokaryotes have the ability to synthesise triacylglycerols, and the process has been studied intensively in plants and animals especially. Many cell types and organs have the ability to synthesise triacylglycerols, but in animals the liver, intestines and adipose tissue are most active with most of the body stores in the last. Within all cells, even thos...

The separation of saturated fatty acids (as the methyl esters and as triacylglycerols) of differing chain lengths by silver ion chromatography (ChromSpher Lipids: containing silver ions: Chrompack, Middelburg, Netherlands) was studied, The retention characteristics (isocratic solvent system of acetonitrile in hexane) of saturated fatty acid methyl esters and homogeneous, saturated triacylglycer...

In our previous studies, medium- and long-chain triacylglycerols (MLCT), randomly interesterified triacylglycerols containing medium-chain and long-chain fatty acids in the same glycerol molecule, significantly reduced body fat accumulation in humans and rats. To clarify mechanism(s) for this effect of MLCT, we measured energy expenditure and hepatic fatty acid metabolism in rats by comparison ...

Diacetyl long-chain 1(3)- and 2-acyl-sn-glycerols containing either [9,10-3H]oleic acid or [1-14C]palmitic acid were synthesized by partial hydrolysis of the corresponding labelled triacylglycerols and acetylation. They were obtained in a high degree of stereochemical purity by preparative h.p.l.c. on a column containing a diol bonded phase. Each compound was rapidly metabolized by adipocyte pr...