Treponema paraluiscuniculi is the causative agent of rabbit venereal spirochetosis. It is not infectious to humans, although its genome structure is very closely related to other pathogenic Treponema species including Treponema pallidum subspecies pallidum, the etiological agent of syphilis. In this study, the genome sequence of Treponema paraluiscuniculi, strain Cuniculi A, was determined by a...

A strain of Spirochoeta refringens has been obtained in pure culture, and its morphological and cultural characteristics have been studied. The strain possessed no pathogenicity for rabbits or monkeys. It probably belongs to the genus Treponema, but it is easily differentiated from Treponema pallidum, Treponema microdentium, and Treponema macrodentium. Its relation to Spirochoeta balanitidis an...

By a method of cultivation that proved successful with Bacterium pneumosintes, characteristic surface colonies of old saprophytic strains of Treponema pallidum and Treponema calligyrum have been obtained. The presence of two types of colony in the pallidum cultures requires explanation, but the fact of colony formation may point the way to new methods of isolation and identification and to the ...

Attempts to devise a vaccine against syphilis have been pursued for decades but thus far with little success. For the most part investigations have been focused on Treponema pallidum, modified in various ways, on the Reiter treponeme and its protein fractions, and on the closely related Borrelia organism. Inoculation with Treponema pallidum produces a degree ofimmunity to re-infection and Neiss...

Experiments carried out on rabbits infected with Treponema pallidum showed that there was a constant invasion and localization of the organisms in the superficial lymph nodes, that the infection persisted indefinitely, and that organisms could be recovered at any time from such nodes as the popliteals. Based upon these observations, a method is proposed for the preservation or recovery of stock...

The distribution of unengorged larvae of Leptotrombidium pallidum, L. fuji and L. kitasatoi in and around 12 rodent-nest holes in Oita Prefecture, Japan was studied using the Tullgren funnel apparatus. Soil was taken from each nest hole, and the ground-surface soil and litter from the surrounding area A (an inner quadrate of 20 cm x 20 cm except the nest hole), and also from the outer area B (a...

An immunofluorescence technique for demonstrating Treponema pallidum in fixed films made from suspected chancre exudate is described. The method used is basically a reversal of the fluorescent treponemal antibody absorption test. It makes use of the fact that by using a syphilitic serum from which group antibody has been absorbed by an extract of Reiter treponemes, leaving only specific antibod...

To date, tissue sections prepared from Formalin-fixed tissues have not been successfully stained with Treponema pallidum subspecies-specific antibody in a direct fluorescent-antibody assay. While current methods stain T. pallidum, they do not distinguish T. pallidum from other spirochetes such as Borrelia burgdorferi (E. F. Hunter, P. W. Greer, B. L. Swisher, A. R. Simons, C. E. Farshy, J. A. C...