Abstract Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Eli...

The present study deals with the establishment of rolA-transgenic and rolB-transgenic plants for first time through Agrobacterium tumefaciens mediated transformation, exploiting inherent morphogenic potential an important medicinal plant, Bacopa monnieri (L.) Wettst. showed integration expression rolA rolB genes respectively, whereas Ri-transformed rolA, rolB, rolC rolD genes. Morphogenic diffe...

The engineering of transgenic canola (Brassica napus L. ) to make tolerance to the broad-spectrum herbicide, glyphosate, is one of the most effective approaches for weed management. Glyphosate inhibits the enzyme EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) enzyme which functions in the shikimate pathway and has a key role in biosynthesis of aromatic amino acids required for survival of ...

the engineering of transgenic canola (brassica napus l. ) to make tolerance to the broad-spectrum herbicide, glyphosate, is one of the most effective approaches for weed management. glyphosate inhibits the enzyme epsps (5-enolpyruvylshikimate-3-phosphate synthase) enzyme which functions in the shikimate pathway and has a key role in biosynthesis of aromatic amino acids required for survival of ...

abstract background: selectable marker gene (smg) systems are critical for generation of transgenic crops. transgenic crop production background: selectable marker gene (smg) systems are critical for generation of transgenic crops. transgenic crop production without using smg is not economically feasible. however, smgs are non-essential once an intact transgenic plant has been established. elim...

an endochitinase gene (chit36) was isolated from the biocontrol fungus trichoderma atroviride. chit36 was overexpressed under the camv35s constitutive promoter in canola. transformation of cotyledonary petioles was achieved via agrobacterium tumefaciens. the insertion of transgene was verified by pcr and dna dot blotting. rt-pcr analysis indicated that the transgenic canola was able to express ...

A significant portion of the world’s cultivated land is affected by salinity that reduces crop productivity in these areas. Breeding for salt tolerance is one of the important strategies to overcome this problem. Recently, genetic engineering is becoming a promising approach to improving salt tolerance. In order to improve the yield performance of canola in saline soils, we transformed canola w...

to investigate the impact of overexpression of atnrt2.1 transgene from arabidopsis on nitrate uptake rate and to understand the regulation of endogenous hats by nitrate and glutamine amino acid (gln) in tobacco plants, wild-type and transgenic (f line) plants grown on soil for 4 weeks were transferred to hydroponic culture in a controlled-environment with a 16/8h l:d photoperiod at 24? c/20...

Background: Newcastle disease is a major avian disease that causes enormous economic loss in poultry industry. There have been a number of reports on the suitability of plant-based recombinant vaccine against this disease. Fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle disease virus (NDV) represent the major immunogenic sites for development of recomb...

cell expansion and cell division are two main processes in plant growth and development. expansin proteins play a role key in cell expansion. these non-enzyme proteins involve in many growth and developmental processes. this protein affects drought tolerance. atexpb2 gene belongs to these proteins family. atexpb2 gene mainly expresses in the root tissue. in this study the expb2 gene, that was i...