through an application of genetic engineering, genes from a soil bacterium bacillus thuringiensis (bt) which confer resistance to pests were transferred into rice. successful employment of genetic engineering in traditional breeding programs is dependent upon stable transmission of transgene in transgenic varieties. three different segregating f2 populations were developed from a cross between ...

cotton cultivar coker has been already transformed with recombinant pbi121-chi via agrobacterium tumefaciens. the t-dna region of pbi121-chi carries the chitinase (chi ) gene from bean and is under the control of the camv35s promoter. t1 and t2 progenies of transgenic cotton containing the chi gene were used in this study.  polymerase chain reaction (pcr), southern and western blotting data con...

an endochitinase gene (chit36) was isolated from the biocontrol fungus trichoderma atroviride. chit36 was overexpressed under the camv35s constitutive promoter in canola. transformation of cotyledonary petioles was achieved via agrobacterium tumefaciens. the insertion of transgene was verified by pcr and dna dot blotting. rt-pcr analysis indicated that the transgenic canola was able to express ...

background: a highly efficient genetic transformation system is essential for a successful genetic manipulation of the african violet (saintpaulia ionantha wendl.).objectives: developing a particle bombardment-based genetic transformation system for the african violet.materials and methods: a local cultivar of the african violet from guilan province was used for transformation experiments. the ...

BACKGROUND: Nicotiana tobacco contains large amounts of alkaloid nicotine. Tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. Reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. Utilizing the new ...

Fusarium wilt caused by Fusarium oxysporum f.sp. Lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. In order to increase the tolerance to this disease, a triple structure containing PR1, chitinase and glucanase genes controlled by 35S promoter was transferred to tomato. Eight days after planting on pre-culture me...

the main aim of this study was to obtain the crimean-congo hemorrhagic fever virus (cchfv) glycoprotein, through either stable transgenic plants or using a transient expression system, and determine the yield, quality and finally the immunogenicity of the plant-made cchfv glycoprotein in a mouse model. we designed and synthesized a codon-optimized g1/g2 gene from the g1 and g2 parts of the cchf...

Paulownia tomentosa (Thunb.) Steud. is a very important hard woody plant, an extremely fast-growing tree and produce timber. Therefore, there is a demand to produce transgenic Paulownia plant resistant to bacterial infection. Microbial infection (especially bacterial one) is serious sever and cause a loss in plant productivity as they bear upon the character and amount of plan...

fusarium wilt caused by fusarium oxysporum f.sp. lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. in order to increase the tolerance to this disease, a triple structure containing pr1, chitinase and glucanase genes controlled by 35s promoter was transferred to tomato. eight days after planting on pre-culture medium, exp...