نتایج جستجو برای: transformation efficiency

تعداد نتایج: 599784  

2016
Sheng-Chun Yu Alexander Dawson Alyssa C. Henderson Eloise J. Lockyer Emily Read Gayathri Sritharan Marjah Ryan Mara Sgroi Pok M. Ngou Rosie Woodruff Ruifeng Zhang Travis Ren Teen Chia Yu Liu Yiyu Xiang Pietro D. Spanu

Efficiency of yeast transformation is determined by the rate of yeast endocytosis. The aim of this study was to investigate the effect of introducing amino acids and other nutrients (inositol, adenine, or p-aminobenzoic acid) in the transformation medium to develop a highly efficient yeast transformation protocol. The target of rapamycin complex 1 (TORC1) kinase signalling complex influences th...

Background: Oat (Avena sativa) with high nutritive value and fiber content is used as the main food grain in many countries for human diet as well as animal feed. Recently, it became difficult to transfer new genes through the conventional breeding due to the lack of desirable traits. Objectives: The current study aimed at achieving a st...

2015
Gavin Lim Daisy Lum Betina Ng Christine Sam

Calcium chloride is commonly included in buffers used to generate chemocompetent bacterial cells. The mechanism that underlies the uptake of exogenous DNA into bacteria is not completely understood. Previous studies have suggested that calcium ions may function to neutralize electrostatic repulsion between DNA and membrane proteins, thereby allowing exogenous DNA to enter membrane pores. Other ...

Journal: :journal of sciences, islamic republic of iran 2010
h. rahnama

agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. this gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. in the present work, we studied maize transformation using a. tumefaciens by identifying som...

2017
Huimin Zhao Zilong Tan Xuejing Wen Yucheng Wang

Syringe infiltration is an important transient transformation method that is widely used in many molecular studies. Owing to the wide use of syringe agroinfiltration, it is important and necessary to improve its transformation efficiency. Here, we studied the factors influencing the transformation efficiency of syringe agroinfiltration. The pCAMBIA1301 was transformed into Nicotiana benthamiana...

2010
HAMID RASHID ASMA AFZAL M. HAROON KHAN ZUBEDA CHAUDHRY SALMAN A. MALIK Mohammad Ali Jinnah

In the present study high efficiency Agrobacterium tumefaciens mediated transformation system of wheat (Triticum aestivum L.) cv. Inqilab-91 was developed by exploiting bacterial culture density and acetosyringone concentration. Agrobacterium strain EHA101 harboring binary vector pIG121Hm, containing gene for GUS activity and hygromycin resistance was used in transformation experiments. Differe...

2010
Ruyu Li Rongda Qu

Switchgrass is one of the most important biomass/bioenergy crops. For its improvement as a feedstock through biotechnological approach, we have developed a high throughput Agrobacterium-mediated transformation system for cv. Alamo and two new elite cultivars, Performer and Colony. Highly regenerable and transformation-competent embryogenic calli were identified and used for genetic transformati...

2016
Qiang Tu Jia Yin Jun Fu Jennifer Herrmann Yuezhong Li Yulong Yin A. Francis Stewart Rolf Müller Youming Zhang

Bacterial competent cells are essential for cloning, construction of DNA libraries, and mutagenesis in every molecular biology laboratory. Among various transformation methods, electroporation is found to own the best transformation efficiency. Previous electroporation methods are based on washing and electroporating the bacterial cells in ice-cold condition that make them fragile and prone to ...

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