Abstract Root-knot nematodes ( Meloidogyne spp.) have been reported to be responsible for large economic losses of agricultural crops due their wide host range and variety suitable climates. The control measures these parasitic depend upon synthetic nematicides a small number bio-based products. Chemical are eliciting adverse effects on the environment human health. In present study, an alterna...

CRISPR/Cas12a-based detection is a novel approach for the efficient, sequence-specific identification of viruses. Here we adopt use CRISPR/Cas12a to identify tomato brown rugose fruit virus (ToBRFV), new and emerging tobamovirus which causing substantial damage global industry. Specific CRISPR RNAs (crRNAs) were designed detect either ToBRFV or closely related mosaic (ToMV). This technology ena...

The aim of this study is to identify and update the list viral pathogens in tomato seeds, which plays an important role for our country's economy, control, on behalf country, factors like TYLCV PepMV, were thought not be existing past but determined by new sensitive methods recent years. It also aim, if PepMV are found, share findings with necessary institutions needs tested, quarantine lists. ...

Tomato mottle mosaic virus (ToMMV) is an emerging seed-transmissible tobamovirus that infects tomato and pepper. Since the first report in 2013 Mexico, ToMMV has spread worldwide, posing a serious threat to production of both crops. To prevent this virus, early accurate detection infection required. In study, we developed method for based on reverse-transcription loop-mediated isothermal amplif...

In contrast to previous observations on phloem-limited geminiviruses supported in movement andaccumulation by RNA viruses such as cucumo- and tobamoviruses, tissue infiltration by Abutilon mosaic virus (AbMV) was enhanced by neither Tobacco mosaic virus nor Tomato mosaic virus (ToMV) in two different hosts, Nicotiana benthamiana and tomato. Both tobamoviruses exerted a negative effect on the DN...

The P30 movement protein (MP) of tomato mosaic tobamovirus (ToMV) is synthesized in the early stages of infection and is phosphorylated in vivo. Here, we determined that serine 37 and serine 238 in the ToMV MP are sites of phosphorylation. MP mutants in which serine was replaced by alanine at positions 37 and 238 (LQ37A238A) or at position 37 only (LQ37A) were not phosphorylated, and mutant vir...

Abstract Tomato mottle mosaic virus (ToMMV) is a tobamovirus found in Solanum lycopersicum sample collected Mexico 2009. To assess the possible presence of ToMMV Europe, accessions from historic seed collection were tested by real-time RT-PCR and Illumina sequencing. was identified historical produced France, Netherlands Spain. Three different near complete genome sequences obtained, each corre...