In this study, we analyzed the influence of two nested genes (p19 and p22) of tomato bushy stunt virus (TBSV) on disease symptoms in systemically infected plants and in local lesion hosts. The contribution of individual genes was determined by bioassays with an infectious clone of wild-type TBSV, with p19/p22 mutant derivatives, and by expression of individual TBSV genes from a heterologous pot...

Tomato bushy stunt virus particles have previously been physically and chemically well characterized. Different strains, however, are known to have different host plants and symptoms, and they can be distinguished serologically. In an attempt to correlate this behavior with physicochemical properties, we have performed a comparative study on isolates of four of the main variants of this virus (...

The motional state of RNA in tomato bushy stunt rirlls, both in thr crystalline st,attx and in solution, has been invrstigat,ed IuGng 31P nuclear magnetic resonance methods. It, has been found that t’hr RNA is highly immobile in tho nativ Crus. and it is suggested that t,he lack of a high-resolution X-ray diffraction patt,crn for either the RNB or the N-terminal repions of the pro&n coat molcrn...

Serological cross-reactions among eighteen virus isolates of the tombusvirus group were compared in precipitin tube and immunodiffusion serological tests. The isolates were also compared by immunoelectrophoresis in agar gel. Although precipitin tube tests showed considerable and reproducible differences between the various isolates, the results were too greatly affected by other factors to be o...

background: the potential of plants as a safe and eukaryotic systems, are considered for producing recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (ptgs) response in this new technology. the use of viral suppressors of gene silencing can prevent ptgs and improve transient expression level of for...