Abstract Bamboo leaves extract (BLE) has a variety of physiological functions such as antitumour, anti-inflammatory, antioxidant and blood fat reduction activities the flavonoids bamboo are major active constituents. To profile in complex BLE, rapid sensitive analytical method based on ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight ta...

Broberg, S. Studies of Oligoand Polysaccharides by MALDI-TOF and ESI-IT MS Mass Spectrometry. Doctor’s dissertation. ISSN 1401-6249, ISBN 91-576-6465-X This thesis presents methods for analyzing carbohydrates with matrix-assisted laser desorption/ionization time-of-flight, MALDI-TOF, and electrospray ionization ion trap multiple-stage, ESI-IT MS, mass spectrometry, MS. MALDI-TOF MS was employed...

Background: It is believed that the loading value of anticancer drug conjugated to the monoclonal antibody, called drug-to-antibody ratio (DAR), is the main quality feature of antibody-drug conjugates. Methods: In this study, matrix assisted laser desorption/ionization mass spectrometry was used to determine the average molecular weight of trastuzumab and its three conjugated forms. The differe...

Poly(ethylene glycol) (PEG) is an inexpensive, commercially available polymer that has many biomedical and material applications. Due to its water-solubility biocompatibility, it been used in drug conjugation, as a humectant, anti-foaming agent food, stabilizer for preservation of antique wooden objects. Despite the robust applications PEG, products oxidative degradation are not fully understoo...

We describe a new interface for a prototype quadrupole-quadrupole-time-of-flight (TOF) mass spectrometer (Centaur, Sciex) that allows rapid switching between electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) modes of operation. Instrument performance in both modes is comparable (i.e., resolution approximately 10,000 FWHM, mass accuracy <10 ppm, sensitivity ap...

The Escherichia coli small (30S) ribosomal subunit is a particularly well-characterized model system for studying in vitro self-assembly. A previously developed pulse-chase monitored by quantitative mass spectrometry (PC/QMS) approach to measuring kinetics of in vitro 30S assembly suffered from poor signal-to-noise and was unable to observe some ribosomal proteins. We have developed an improved...

trescine are major polyamines in mammalian cells, and they play important roles in cell growth and differentiation. Although a variety of analytical methods for polyamines have been developed, there is still a need for a simpler, more sensitive and accurate method. The mass spectrometric method can simultaneously separate and identify polyamines on the basis of the difference of their mass with...

Algal biotoxins, chemical compounds produced by some microscopic algae, constitute the phytoplankton. The mussels, feeding on phytoplankton, can accumulate these compounds to become themselves toxic. There have been several cases of food poisoning by consumption of contaminated shellfish. Such food poisoning have pushed our health care system to provide monitoring of shellfish in the framework ...

With 2-D gel mapping, it is often observed that essentially identical proteins migrate to different positions in the gel, while some seemingly well-resolved protein spots consist of multiple proteins. These observations can undermine the validity of gel-based comparative proteomic studies. Through a comparison of protein identifications using direct MALDI-TOF/TOF and LC-ESI-MS/MS analyses of 2-...

A combination of LC and MS was applied to an isogenic breast tumor metastasis model to identify proteins associated with a cellular phenotype. Chromatofocusing followed by nonporous-RP-HPLC/ESI-TOF MS was applied to cell lysates of a pair of monoclonal cell lines from the human breast carcinoma cell line MDA-MB-435 that have different metastatic phenotypes in immune-compromised mice. This metho...