Natural isolates of Tobacco mild green mosaic virus (TMGMV) fail to infect tomato because the tomato tm-1 protein binds to the replication proteins of TMGMV and prevents RNA replication. Previously, we isolated a TMGMV mutant that overcomes tm-1-mediated resistance and multiplies in tomato plants. Here, we show that the causal mutations in the replication protein gene that abolish the interacti...

SUMMARY Taxonomy: Turnip vein-clearing virus (TVCV) is a member of subgroup 3 of the Tobamovirus genus and is thus a member of the alphavirus-like supergroup of positive sense RNA-containing viruses. Physical properties: Virions, typical of tobamoviruses, are rod-shaped and consist of a single species of four-helix bundle capsid proteins of 17 kDa helically arranged around a 6.3 knt RNA which a...

In the early days of molecular farming, Agrobacterium-mediated stable genetic transformation and the use of plant virus-based vectors were considered separate and competing technologies with complementary strengths and weaknesses. The demonstration that 'agroinfection' was the most efficient way of delivering virus-based vectors to their target plants blurred the distinction between the two tec...

Post-transcriptional gene silencing (PTGS) is an important mechanism for regulation of plant gene expression and virus-plant interactions. To better understand this process, the heavy metal cadmium was identified as a specific inhibitor in two different PTGS systems, constitutive and inducible. The pattern of cadmium-induced inhibition of PTGS allowed several insights into PTGS development. Fir...

The reaction of pepper breeding lines under organic production conditions to Verticillium wilt, viruses and phytoplasma was observed at Maritsa Vegetable Crop Research Institute, Plovdiv, Bulgaria during two successive years. diseases severity recorded, when symptoms dahliae, Phytoplasma were deeply expressed. dahliae attack index for early varied widely from 15.04% line O58/14 43.83% O26/16. F...