objective(s):osteoarthritis (oa) is globally one of the most common diseases from the middle age onwards. cartilage is an avascular tissue therefore it cannot be repaired in the body. conservative treatments have failed as a good remedy and cell therapy as a decisive cure is needed. one of the best and easily accessible cell sources for this purpose is adipose-derived stem cells which can be di...

The production of transfected fish embryos requires expertise in injecting the fertilized eggs and/or expensive equipment for electroporation or microprojectiles. This article demonstrates that by exposure to DNA constructs conjugated with transfecting reagents dechorionated Danio rerio embryos are capable of acquiring extracellular DNA and expressing reporter genes. Embryos incubated with pCMV...

ALEIS CARREL was one of the pioneers of tissue culture and its chief publicist. He was largely responsible for the early development of the technique, but although he made a number of practical contributions, it was his influence on his contemporaries that was particularly significant. Carrel's tissue culture techniques were based on his surgical expertise and they became increasingly complicat...

The protein-stabilizing and cell-penetrating activities of Bombyx mori 30Kc19 α-helix domain (30Kc19α) are investigated. Recently, 30Kc19 protein has been studied extensively as it has both protein-stabilizing and cell-penetrating properties. However, it is unknown which part of 30Kc19 is responsible for those properties. 30Kc19 protein is composed of two distinct domains, an α-helix N-terminal...

This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing.

aflatoxins (afs) are one of the most important mycotoxins due to their common occurrence in feedstuffs and feeds that pose a serious threat to humans and animals. although many outbreaks of acute and chronic diseases have been attributed to consumption of aflatoxin-contaminated foods, the most significant effect of aflatoxin is hepatotoxicity in farm animals, especially aflatoxicosis in shrimps...