The protein isolated from beef pancreas (1) was recently identified as the zymogen of a proteolytic enzyme and provisionally called protein B (2). In this paper a method of crystallization of the activated enzyme is described. Further properties of this enzyme have been studied, suggesting that the activated protein B belongs to the group of chymotrypsins. The strongest evidence for this conclu...

A global kinetic analysis of a general zymogen activation model, where not only the activating but also the activated enzyme suffer an irreversible inhibition is presented. A reaction in which the enzyme acts upon a substrate is coupled to monitor the process. In addition, we determined the corresponding kinetic equations for a number of particular cases of the general model studied. Finally, a...

we propose a novel approach for detecting precursors to epileptic seizures in intracranial electroencephalograms (ieeg), which is based on the analysis of system dynamics. in the proposed scheme, the largest lyapunov exponent of the discrete wavelet packet transform (dwpt) of the segmented eeg signals is considered as the discriminating features. such features are processed by a support vector ...

Prostaphopain B is the precursor of staphopain B, a papain-type secreted cysteine protease from the pathogen Staphylococcus aureus. Here, we describe the 2.5 A crystal structure of the proenzyme. Its 21 kDa proregion is organized around a central half-barrel or barrel-sandwich hybrid and occludes primed, but not nonprimed, sites in the active site cleft of the protease. The structure of the mat...

in this article, hybrid organic-inorganic materials were prepared by free radical copolymerization of 2-hydroxyethyl methacrylate (hema) and 3-methacryloxy-propyltrimethoxysilane (mptms) via sol-gel process. the methoxysilane-containing copolymer precursors were synthesized at different compositions. these copolymer precursors were then hydrolyzed and condensed to produce phema-sio2 nanocomposi...