A novel strategy for a simultaneous multianalyte electrochemical immunoassay is proposed using thionine-labeled anti-AFP and ferrocene-labeled anti-CEA as tracers; each biorecognition event yielded a distinct voltammetric peak, whose position and size reflected the identify and level of the corresponding antigen.

Anew, highly sensitive and selective catalytic kinetic method (CKM) for determination of osmium (VIII) has been established based on its catalytic effect on the oxidation of thionine dye by hydrogen peroxide in alkaline medium. The reaction has been followed spectrophotometrically by measuring the decrease in the absorbance band of thionine at 600 nm. Under the optimum conditions, the proposed ...

Within 3.5 to 4 hours after thionine administration, numerous small osmiophilic bodies, liposomes, appear in the endoplasmic reticulum of the liver cells. By fusion, the liposomes lead to the formation of larger collections of fat, giant liposomes. Adenine administration to ethionine-treated rats removes the liposomes from the hepatocytes and causes the transitory appearance of osmiophilic drop...

Absolute radical yields, as determined by flash spectroscopy, are reported for the reaction of the thionine triplet 3TH+ and its protonated form 3TH2++ with a variety of electron donors in methanolic solution. The different yields, extrapolated to infinite donor concentrations, are due to partial physical quenching of the triplets in the electron transfer reaction. The results are indicative of...

The enzyme dans metbylase which recognizes and methylates the adenine in the palindromic sequewrce OATC in DNA was isolated and the secondary structure was determixzcd by CD spectroscopy and various predicting methods from the amino acid sequence. The interaction of &rn methylase with S-adenosylmew thionine was studied by CD spectr~scupy indicating a decrease of the: percentage of E-helix as th...

Methylene blue (MB) and toluidine blue (TB) when administered in maximum tolerated oral doses to mice and to cotton rats are highly effective in preventing the usual lethal outcome of intraperitoneally induced infections with R. orientalis. This activity is manifest even when dye administration is delayed until a systemic infection has been well established. Methylene blue is also effective in ...