We have developed a technique to manipulate bifunctional DNA molecules: One end is thiolated to bind to a patterned gold surface and the other end is biotinylated to bind to a microtubule gliding over a kinesin-coated surface. We found that DNA molecules can be stretched and overstretched between the gold pads and the motile microtubules, and that they can form dynamic networks. This serves as ...

Chitosan is a natural polycationic copolymer consisting of glucosamine and N-acetylglucosamine units. The polymer has valuable properties as a biomaterial because it is considered to be biocompatible, biodegradable and non-toxic. The purpose of this review article is to provide detailed information about thiolated chitosans which are gaining popularity because of their high mucoadhsiveness and ...

A surface plasmon resonance sensor for Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) is developed using repeatable telecommunication wavelength modulation based on optical fiber communications laser wavelength and stability. MTB DNA concentrations of 1 μg/mL and 10 μg/mL were successfully demonstrated to have the same spectral half-width in the dip for optimum coupling. The sensi...

Thiol functionalization of psyllium was carried out to enhance its mucoadhesive potential. Thiolation of psyllium was achieved by esterification with thioglycolic acid. Thiolation was observed to change the surface morphology of psyllium from fibrous to granular and result in a slight increase in the crystallinity and swelling. Thiolated psyllium was found to contain 3.282 m moles of thiol grou...

sanger, and amino acid transfer of the macromolecules. The inhibitory effects of partially Ihiobated polynucleotides on nucleic acid polymarases from Micrococcus !ysodeikti cus (i6), RNA humor viruses (7, 8), and cultured Bunkitt lymphoma calls (21) have been described. Recent studies revealed that the partially thiolated polynucleohides can en tar tumor cells and are transported into the nucle...

Oral chemotherapy is a key step towards 'chemotherapy at home', a dream of cancer patients, which will radically change the clinical practice of chemotherapy and greatly improve the quality of life of the patients. In this research, three types of nanoparticle formulation from commercial PCL and self-synthesized d-α-tocopheryl polyethylene glycol 1000 succinate (PLA-PCL-TPGS) random copolymer w...

An experimental approach for improving the sensitivity of the surface plasmon resonance (SPR) DNA hybridization sensor using gold nanoparticles (GNPs), modified by specific oligonucleotides, was elaborated. An influence of the ionic strength on the aggregation stability of unmodified GNPs and GNPs modified by the thiolated oligonucleotides was investigated by monitoring a value of light extinct...

Thiols have a strong tendency to be chemisorbed to gold surfaces forming self-assembled monolayers. 2] The feature of strong S Au bond formation has been exploited in numerous applications including the immobilisation of DNA on gold surfaces for the generation of DNA microarrays or gold electrodes for measuring charge migration mediated by double stranded DNA. To be immobilised on gold, DNA has...

The successful development of multifunctional drug delivery systems is mainly based on the development and evaluation of new mucoadhesive polymers. Chitosan is a natural polycationic copolymer consisting of glucosamine and Nacetylglucosamine units. The polymer has valuable properties as a biomaterial because it is considered to be biocompatible, biodegradable and non-toxic. The derivatization o...

We report here an impedimetric genosensor for the quantification of microRNA-21 using [Fe(CN)6]3?/4? as redox probe to transduce hybridization event. The biosensing platform was built at a thiolated-gold electrode by covalent bond reduced graphene oxide (RGO) modified with chitosan (CHIT) and further attachment aminated DNA probe. GO used provide carboxylic groups CHIT and, once reduced, improv...