Fungus growth methods for estimating either vitamin B1 thiazole or vitamin B1 pyrimidinel have been used by Robbins and Kavanagh (l), Kavanagh (2), and Krampitz and Woolley (3), and a fermentation stimulation method has been described by Schultz, Atkin, and Frey (4). A procedure utilizing the synthesis of vitamin B1 by yeast from thiazole and pyrimidine has been used in this laboratory to estim...

The crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared as a rich source of peroxidase and its enzymatic and specific activities in the presence of cross-linked polyvinylpyrrolidone as a stabilizer were determined. This extract was used to catalyze the oxidation of thiamine in the presence of hydrogen peroxide to thiochrome at pH 8.0. Thiochrome shows strong fluorescence at 43...

background and purpose: mycotoxins are secondary fungal metabolites with a very high diversity that are produced by some species of aspergillus which frequently leads to contaminate food and agricultural products. recently, elimination of aflatoxin contamination in food and feed has been considered by scientists worldwide. although, the antibacterial and antifungal effects of vitamins as natura...

Kromatografi pasangan ion dapat digunakan untuk meretensi senyawa yang memiliki muatan, bersifat polar dan memisahkan analit bervariasi kepolaran hidrofobisitasnya. Hal tidak dilakukan dengan mudah pada metode kromatografi fase balik biasa. Pemisahan kolom menggunakan menganalisa kadar Thiamine Mononitrat (Vitamin B1), Pyridoxine HCl B6) Cyanocobalamin B12) secara simultan. Validasi juga membuk...

thiamine responsive megaloblastic anemia (trma), also known as roger syndrome is an autosomal recessive disorder resulting from the deficiency of thiamine (vitamin b1) transporter protein. this is the report of a 3- year follow up of a female child who presented in 2000 at the age of 11 with severe anemia, congenital deafness and diabetes mellitus. in our follow-up period we prescribed 100-mg t...

We describe optimized, ultraviolet spectrophotometric procedures for determination of erythrocyte transketolase, glutathione reductase, and aspartate aminotransferase activity, and their activation by their respective coenzymes--thiamine pyrophosphate, flavin-adenine dinucleotide, and pyridoxal-5-phosphate--as tests for vitamin B1, B2, and B6 deficiency. With these procedures we have investigat...

Acupuncture has long been used to treat urticaria in the Asian world. Acute urticaria can be easily and effectively treated with acupuncture. L111 (Quchi), Sp10 (Xuehai), Sp6 (Sanyinjiao), and S36 (Zusanli) are the 4 acupuncture points most commonly prescribed. Chronic urticaria is a challenge for medical therapy. There are at least 6 kinds of acupuncture methods developed to overcome this chal...

The microbiota of Drosophila melanogaster has a substantial impact on host physiology and nutrition. Some effects may involve vitamin provisioning, but the relationships between microbe-derived vitamins, diet, and host health remain to be established systematically. We explored the contribution of microbiota in supplying sufficient dietary thiamine (vitamin B1) to support D. melanogaster at dif...

Background and Purpose: Mycotoxins are secondary fungal metabolites with a very high diversity that are produced by some species of Aspergillus which frequently leads to contaminate food and agricultural products. Recently, elimination of aflatoxin contamination in food and feed has been considered by scientists worldwide. Although, the antibacterial and antifungal effects of vitamins as natura...