An extracellular xylanase from the thermophilic fungus Melanocarpus albomyces IIS 68 was evaluated for its activity and stability in the presence of polyols and salts at 60 °C, and found to be an effective protecting agent for thermal deactivation of enzyme. Response surface methodology was employed to study the synergistic effects of glycerol and NaCl (thermostabilizers) for xylanase stability...

mesophilic bacteria, fungi and actinomycetes varied during composting cycles with high numbers in the initial and final cycles with maximal values in compost c and d, and sharply decreased in the heating cycles. staphylococcus aureus and bacillus subtilis were dominated at the initial composting cycle. whereas, at maturity bacillus subtilis was the major followed by b.badies, b.polymixa and b.b...

1. Glucose 6-phosphate dehydrogenase was isolated and partially purified from a thermophilic fungus, Penicillium duponti, and a mesophilic fungus, Penicillium notatum. 2. The molecular weight of the P. duponti enzyme was found to be 120000+/-10000 by gelfiltration and sucrose-density-gradient-centrifugation techniques. No NADP(+)- or glucose 6-phosphate-induced change in molecular weight could ...

BACKGROUND Over the past 3 years, the CRISPR/Cas9 system has revolutionized the field of genome engineering. However, its application has not yet been validated in thermophilic fungi. Myceliophthora thermophila, an important thermophilic biomass-degrading fungus, has attracted industrial interest for the production of efficient thermostable enzymes. Genetic manipulation of Myceliophthora is cru...

Thermophilic fungi are commonly found in self-heated habitats, such as composts, and help to decompose various plant materials. However, there have been few reports describing production of biologically active substances by these fungi. During a screening programme for new antibiotics produced by thermophilic microorganisms, a substance with activity against Gram-positive and strictly anaerobic...

A novel filamentous fungus strain designated RB-1 was isolated into pure culture from Japanese rice field soil through an anaerobic role tube technique. The strain is a mitosporic fungus that grows in both aerobic and strict anaerobic conditions using various mono-, di-, tri-, and polysaccharides with acetate and ethanol productions. The amount of acetate produced was higher than that of ethano...

This short paper reviews two groups of enzymes designated as polyethylene terephthalate (PET) hydrolases: one consists thermophilic cutinases from microorganisms (actinomycetes and a fungus) the other mesophilic cutinases, representative which is IsPETase bacterium. From viewpoint that PET hydrolysis requires high temperature close to glass transition (65–70 °C in water) PET, are not suitable f...