Bausum, Howard T. (Fort Detrick, Frederick, Md.), and Thomas S. Matney. Boundary between bacterial mesophilism and thermophilism. J. Bacteriol. 90:50-53. 1965.-The temperature boundary between bacterial mesophilism and thermophilism has been identified as 44 to 52 C. Facultative thermophiles growing in the mesophilic range require a brief period of adaptation at intermediate temperatures before...

Our understanding of thermophile diversity is based predominantly on PCR studies of community DNA. "Universal" and domain-specific rRNA gene PCR primers have historically been used for the assessment of microbial diversity without adequate regard to the degree of specificity of primer pairs to different prokaryotic groups. In a reassessment of the published primers commonly used for "universal"...

The full biotechnological exploitation of enzymes is still hampered by their low activity, low stability and high cost. Temperature-dependent catalytic properties of enzymes are a key to efficient and cost-effective translation to commercial applications. Organisms adapted to temperature extremes are a rich source of enzymes with broad ranging thermal properties which, if isolated, characterize...

Among four thermophilic moulds, Sporotrichum thermophile produced high titres of xylanases, pectinases and cellulases after 4 days of incubation in solid-state fermentation (SSF). Of the 27 different combinations of agro-residues tried, wheat bran (WB) and citrus pectin (CP) in 1:1 ratio supported a very high production of enzymes. When the mixed substrate at pH 7.0 was moistened with tap water...

A spontaneous kanamycin resistance and capreomycin resistance mutation, A1408G, in the decoding center of 16S rRNA, was identified in the extreme thermophile Thermus thermophilus. Unexpectedly, this mutation also confers resistance to streptomycin. We propose a novel mechanism of streptomycin resistance by which A1408G influences conformational changes in 16S rRNA during tRNA selection.

background and objectives: leucine dehydrogenase (leudh; ec 1.4.1.9) belongs to the amino acid dehydrogenase family and isused as a biocatalyst in medical and pharmaceutical industries (1). this study reported deals with the isolation and characterization of leudh from a thermophilic bacterium isolated from jask port in the province of hormozgan. materials and methods: aliquots of soil and wate...