The possibility of thermal lensing of dynamically emerging light-absorbing layers at cell surfaces was investigated. Analyte accumulation at a cell surface determines long-term changes in the thermal-lens signal that was used as a source of analytical information to enhance sensitivity of thermal lensing. Considering the rate of accumulation as an additional analytical signal, we achieved a thr...

The wavelength dependence of thermal lens signal from organic dyes are studied using dual beam thermal lens technique. It is found that the profile of thermal lens spectrum widely differ from the conventional absorption spectrum in the case of rhodamine B unlike in the case of crystal violet. This is explained on the basis of varying contribution of nonradiative relaxations from the excited vib...

In the emerging field of thermoplasmonics, Joule heating associated with optically resonant plasmonic structures is exploited to generate nanoscale thermal hotspots. In the present study, new methods for designing and thermally probing thermoplasmonic structures are reported. A general design rationale, based on Babinet's principle, is developed for understanding how the complementary version o...

Contaminations of the population lens and transient absorption in thc obsened thermal lens signal sometimes cause a misinterpretation of the signal. Rcccntly, we ha1.c developed several methods to evaluate the relative contributions of these components in thc observed thermal lens signal. These methods with reccnt results are sumtnarizcd, and mcrits and limitations of each method are discussed.

introduction: tomographical maps from the serial sections for light microscopy were prepared and reconstructed by autocad software. materials and methods: in this study, the three-dimensional reconstruction method was used and the following results were obtained (see below). results: 1) the surface ectodermal epithelium was thick and invaginated. 2) the invagination of surface ectodemal epithel...

The use of a confocal detection scheme in a dual-beam thermal-lens microscope is presented. The scheme allows the measurement of absorption factors down to 1.2 x 10(-7) in a 0.35-microm3 volume by use of a heating laser power of 100 mW incident upon the sample. Results are presented that prove that a 450-nm axial resolution is possible when a 1.2 water immersion objective lens with a N.A. of 1....