The continuous production and harvest of uncontaminated cells for periods of several months are a major requirement inthe design of a suitable culture system. Many continuous-culture units have been reported previously (1, 2), and some units are now commercially available. However, in these units, rubber, metals, and other sources of potentially toxic extractives are not prevented from direct c...

The American long-t long-t long-t long-t long-term care (L erm care (L erm care (L erm care (L erm care (LT T T T TC) C) C) C) C) system, based on a medical model, regards residents as sick patients, unable to care for themselves. Routines are organized for the efficient operation of the facility, rather than the needs of residents. 1 The focus of care is to treat the residents' weaknesses, not...

We have cloned adherent synovial cells from rheumatoid synovitis. These can be generally divided into three types, including cells that have the characteristic features of dendritic cells (DCs), macrophagelike cells (MCs) and fibroblastlike cells (FCs), as classified by morphology and immunofluorescent staining. The cloned cells were able to divide and were cultured for up to 11 mo without any ...

Pluripotent hematopoietic stem cells (PHSCs) show self-renewal and give rise to all blood cell types. The extremely low number of these cells in primary hematopoietic organs and the lack of culture systems that support proliferation of undifferentiated PHSCs have precluded the study of both the biology of these cells and their clinical application. We describe here cell lines and clones derived...

Acquiring abundant and high-purity cancer stem cells (CSCs) is an important prerequisite for CSC research. At present, researchers usually gain high-purity CSCs through flow cytometry sorting and expand them by short-term sphere culture. However, it is still uncertain whether we can amplify high-purity CSCs through long-term sphere culture. We have proposed a mathematical model using ordinary d...

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) reduction assay is a frequently used and easily reproducible method to measure beta-amyloid (Abeta) toxicity in different types of single cell culture. To our knowledge, the influence of Abeta on MTT reduction has never been tested in more complex tissue. Initially, we reproduced the disturbed MTT reduction in neuron and ast...