capillary electrophoresis (ce) with indirect uv detection is an interesting analytical method for the analysis of drugs and pharmaceuticals. good and reproducible capillary quality is needed to develop robust methods and to facilitate method transfer in ce. it is widely accepted that preconditioning procedures are indispensable in capillary electrophoresis in order to achieve reproducibility of...

High-resolution ion mobility/mass spectrometry methods have been used to examine the influence of solvent composition and capillary temperature on the gas-phase conformations of ubiquitin ions (16 to 113) formed during electrospray ionization. Three general conformer types are observed: compact forms (favored for the 16 and 17 charge states); partially folded conformers (favored for the 18 and ...

Thermal end effects on electroosmotic flow in a capillary are numerically investigated in this paper. The model accounts for the dynamic coupling effects of Joule heating on the temperature field, the electrical double layer field, the applied electric potential field and the flow field in the full capillary from reservoir to reservoir. These fields are strongly coupled via temperature dependen...

The thermal stability of ribonuclease S (RNase S), an enzymatically active noncovalent complex composed of a 2166-u peptide (S-peptide) and a 11,534-u protein (S-protein), was investigated by electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis ESI-MS (CE-ESI-MS). The intensities of peaks corresponding to the RNase S complex were inversely related to both the applied...

Herein we describe a simple platform for rapid DNA amplification using convection. Capillary convective PCR (CCPCR) heats the bottom of a capillary tube using a dry bath maintained at a fixed temperature of 95°C. The tube is then cooled by the surrounding air, creating a temperature gradient in which a sample can undergo PCR amplification by natural convection through reagent circulation. We de...

BACKGROUND AND OBJECTIVES Glycated hemoglobin (HbA1c) provides an accurate and reliable method to assess the glycemic control in patients with Diabetes. Its measurement is limited by the inconvenience of sample collection that requires venipuncture, sample handling and storage factors. The aim of this study was to assess the feasibility of using a dried capillary blood spot on a filter paper to...

A novel capillary tube has been tested and evaluated experimentally in the refrigeration cycle of window type air conditioner. Three twisted capillary tubes have been demonstrated and evaluated to show the performance of these tubes and its ability in the depressurization process seeking for short capillary tube. The effect of twist angle and the number of twisted points on the refrigeration cy...

Abstract Steam-water capillary pressure is often either ignored or determined artificially in geothermal reservoir engineering. However steam-water capillary pressure plays an important role in geothermal reservoir performance and may not be substituted by air-water capillary pressure. To this end, preliminary mathematical models have been developed to calculate drainage and imbibition steam-wa...

The density of 0.5–3 mm thick vapor-deposited films of water ice were measured by combined optical interferometry and microbalance techniques during deposition on an optically flat gold substrate from a capillary array gas source. The films were of high optical quality with an index of refraction of 1.2960.01 at 435.8 nm, a density of 0.8260.01 g/cm, and a porosity of 0.1360.01. In contrast to ...

INTRODUCTION There are no available data addressing the potential clinical risks of open-water swimming competitions. OBJECTIVE Address the risks of hypothermia and hypoglycemia during a 10-km open-water swimming competition in order to alert physicians to the potential dangers of this recently-introduced Olympic event. METHODS This was an observational cross-sectional study, conducted duri...