beta-Xylosidase secreted by the shoyu koji mold, Aspergillus oryzae, is the key enzyme responsible for browning of soy sauce. To investigate the role of beta-xylosidase in the brown color formation, a major beta-xylosidase, XylA, and its encoding gene were characterized. beta-Xylosidase XylA was purified to homogeneity from culture filtrates of A. oryzae KBN616. The optimum pH and temperature o...

Phyllosticta (Phyllostictaceae, Botryosphaeriales) species are widely distributed globally and constitute a diverse group of pathogenic endophytic fungi associated with broad range plant hosts. In this study, four new Phyllosticta, i.e. P. endophytica, jiangxiensis, machili, xinyuensis, were described using morphological characteristics multi-locus phylogeny based on the internal transcribed sp...

Abstract Peppermint is an important medicinal plant, and it known for its essential oils phenolic acids. Verticillium wilt a vascular disease resulted from several spp. causing significant economic losses in peppermint cultivation. In this study, the fungus Gibellulopsis nigrescens (syn. ) was isolated symptomless plants during regular control of on Germany. A pure fungal culture prepared, DNA ...

BACKGROUND Quantitative real-time reverse-transcriptase PCR (qRT-PCR) is an important technique for analyzing differences in gene expression due to its sensitivity, accuracy and specificity. However, the stability of the expression of reference genes is necessary to ensure accurate qRT-PCR assessment of expression in genes of interest. Perennial ryegrass (Lolium perenne L.) is important forage ...

In this study, we introduce a new species Nigrograna wuhanensis, which was collected from Camellia japonica in Hubei Province, China. The established based on morphological characteristics and multi-locus phylogenetic analysis of combined SSU, LSU, ITS tef1-α sequence data. wuhanensis is characterized by scattered, immersed ascomata with long neck superfi cial the substrate, clavate short pedic...

One gene coding for yeast cytoplasmic elongation factor 1 alpha (EF-1 alpha) was isolated by colony hybridization using a cDNA probe prepared from purified EF-1 alpha mRNA. A recombinant plasmid, pLB1, with a 6-kilobase yeast DNA insert, was found by hybrid selection and translation experiments to carry the entire gene. The nucleotide sequence of the gene with its 5'- and 3'-flanking regions wa...

Sustainability of nutrient requirements for microbial proliferation on a large scale is a challenge in bioremediation processes. This article presents data on biochemical properties of a free cyanide resistant and total nitrogen assimilating fungal isolate from the rhizosphere of Zeamays (maize) growing in soil contaminated with a cyanide-based pesticide. DNA extracted from this isolate were PC...

The ascomycete family Nectriaceae (Hypocreales) includes numerous important plant and human pathogens, as well as several species used extensively in industrial and commercial applications as biodegraders and biocontrol agents. Members of the family are unified by phenotypic characters such as uniloculate ascomata that are yellow, orange-red to purple, and with phialidic asexual morphs. The gen...

inflammatory bowel disease (ibd) is a chronic disease of unknown etiology, in which genetic factors, seem to play an important role in the disease predisposition and course. assessment of tumor necrosis factor (tnf-α) gene polymorphisms in many populations showed a possible association with ibd. considering the genetic variety in different ethnic groups, the aim of the present study was to inve...