نتایج جستجو برای: tachyzoites rh strain

تعداد نتایج: 233803  

2011
S. Muñiz-Hernández M. González del Carmen M. Mondragón C. Mercier M. F. Cesbron S. L. Mondragón-González S. González R. Mondragón

Toxoplasma gondii proliferates and organizes within a parasitophorous vacuole in rosettes around a residual body and is surrounded by a membranous nanotubular network whose function remains unclear. Here, we characterized structure and function of the residual body in intracellular tachyzoites of the RH strain. Our data showed the residual body as a body limited by a membrane formed during prol...

2016
Niloofar Mohammadpour Jasem Saki Abdollah Rafiei Ali Khodadadi Mehdi Tavalla Bahman Cheraghian

BACKGROUND Toxoplasma gondii is one of the most common causes of latent infections in humans worldwide. Detecting anti-Toxoplasma antibodies in serum using serological tests is a common method to diagnose toxoplasmosis. OBJECTIVES In the present study, an indigenous ELISA kit was prepared using tachyzoites from the RH strain of T. gondii, and its sensitivity and specificity were compared with...

Journal: :The Korean journal of parasitology 2008
Eun-Hee Shin Dong-Hee Kim Aifen Lin Jo-Woon-Yi Lee Hyo-Jin Kim Myoung-Hee Ahn Jong-Yil Chai

To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands w...

Journal: :iranian journal of parasitology 0
mohey abdel-hafez hassanain department of zoonosis, veterinary research division, national research center, dokki, giza, egypt. eman hussien abdel-rahman department of parasitology and animal diseases, veterinary research division, national research center, dokki, giza, egypt. nagwa ibrahim toaleb department of parasitology and animal diseases, veterinary research division, national research center, dokki, giza, egypt. raafat mohamed shaapan department of zoonosis, veterinary research division, national research center, dokki, giza, egypt. hasan ali elfadaly department of zoonosis, veterinary research division, national research center, dokki, giza, egypt. nawal abdel-hafez hassanain department of zoonosis, veterinary research division, national research center, dokki, giza, egypt.

background: serological diagnosis of toxoplasma gondii infection using crude antigens may not be more accurate. to increase the diagnostic potency of anti-gens, isolation of their immunogenic fractions could be useful. the current re-search adopted to obtain an affinity isolated fraction from rh strain using cnbr sepharose 4b column coupled with infected mice sera helping in detec-tion of igm a...

Journal: :The Southeast Asian journal of tropical medicine and public health 2003
Yaowalark Sukthana Phuangphet Waree Emsri Pongponratn Urai Chaisri Mario Riganti

We studied the pathology of acute toxoplasmosis in experimental mice inoculated with RH strain tachyzoites of Toxoplasma gondii. All died from severe disseminated toxoplasmosis involving the liver, spleen and pancreas. Pathological features of acute toxoplasmosis in susceptible mice could be regarded as an excellent model for acute reactivation of Toxoplasma in the immunosuppressed host.

2013
Saeedeh Shojaee Hossien Keshavarz S Shojaee

Objectives: Toxoplasma gondii is an obligate intracellular parasite that infects a broad range of warmblooded animals including human. Tachyzoites of T.gondii invade the host cell, replicate and finally lead to the lysis of the cell. T. gondii is associated with congenital infection and it can cause encephalitis, or systemic infection in immunocompromised patients. It is important to know wheth...

Journal: :The Journal of infectious diseases 2000
C W Angus D Klivington-Evans J P Dubey J A Kovacs

Immunization with DNA can induce humoral and cell-mediated immune responses, both of which are important in conferring immunity to Toxoplasma gondii. The efficacy of genetic vaccination with a cDNA encoding the T. gondii SAG1 (P30) surface antigen was evaluated. Sera of immunized mice showed recognition of T. gondii tachyzoites by immunofluorescence and exhibited high titers of antibody to SAG1...

Journal: یافته 2009
ali reza Andalib, behroz Ezatpour , behzad Haghpanah, seyed hosein Hejazi, zahra Ghayour,

Taoxoplasma gondii is an important food-borne parasite transmitted primarily from animals to humans through meat consumption, mainly pork and lamb, as well as through oocysts shed by cats. Infection in humans can cause severe fetal malformations, ocular complications or encephalitis. Toxoplasmosis infection during pregnancy, especially in sheep, often results in abortion, representing considera...

Journal: :European review for medical and pharmacological sciences 2012
V R Puvanesuaran K Nowroji S Sreenivasan R Noordin V Balakrishnan

AIM To determine the usefulness of prednisolone in increasing the number of Toxoplasma (T.) gondii tachyzoites and bradyzoites in mice. MATERIALS AND METHODS The mice were water-fasted prior to being immunosuppressed with oral inoculation of prednisolone. Tachyzoites of 7T gondii RH strain were inoculated into mice and the number of the parasites in the intraperitoneal fluids was then determi...

A. Dalimi, F. Ghafari Far K. Solhjoo Z. Sharifi

  Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید