Characteristics of GABAergic and Cholinergic Neurons in the Perinuclear Zone of Mouse 1 Supraoptic Nucleus 2 3 Lie Wang, Matthew Ennis, Gábor Szabó and William E. Armstrong 4 Department of Anatomy and Neurobiology and Neuroscience Institute 5 University of Tennessee Health Science Center 6 Memphis, TN 38163 7 2 Department of Gene Technology and Developmental Biology 8 Institute of Experimental ...

In this study, the P2X(5) receptor was found to be distributed widely in the rat hypothalamus using single and double labeling immunofluorescence and reverse transcriptase-polymerase chain reaction (RT-PCR) methods. The regions of the hypothalamus with the highest expression of P2X(5) receptors in neurons are the paraventricular and supraoptic nuclei. The intensity of P2X(5) immunofluorescence ...

The study was undertaken to investigate the possible correlation of total volume of mitochondria per cell with the rate of succinate oxidation in isolated nerve cell bodies, after various functional stresses in the experimental animals. Significant cytological effects were found in the nerve cells of the supraoptic nucleus in rats which had been thirsting for 4-12 days or had been given 2% sodi...

Argininosuccinate synthetase (ASS) and nitric oxide synthase (NOS) comprise part of the cyclic metabolic pathway to produce nitric oxide (NO). ASS is one of the arginine synthesis enzymes which synthesizes argininosuccinate from aspartate and citrulline, and NOS forms NO and citrulline from arginine. This study examines the localization of ASS and NOS in the cat hypothalamus using nicotinamide ...

The study was undertaken to investigate the possible correlation of total volume of mitochondria per cell with the rate of succinate oxidation in isolated nerve cell bodies, after various functional stresses in the experimental animals. Significant cytological effects were found in the nerve cells of the supraoptic nucleus in rats which had been thirsting for 4-12 days or had been given 2% sodi...

How does a neuron, challenged by an increase in synaptic input, display a response that is independent of the initial level of activity? Here we show that both oxytocin and vasopressin cells in the supraoptic nucleus of normal rats respond to intravenous infusions of hypertonic saline with gradual, linear increases in discharge rate. In hyponatremic rats, oxytocin and vasopressin cells also res...

The alterations in local cerebral glucose utilization in 58 anatomically discrete regions which occur during a period of hemorrhagic hypotension have been investigated in conscious rats, using the quantitative autoradiographic 14C-deoxyglucose technique. Hemorrhagic hypotension (mean arterial pressure reduced by approximately 50 mm Hg) effected significant increases in glucose utilization in ei...

Oxytocin and vasopressin release from magnocellular neurons of the supraoptic nucleus is under the control of glutamate-dependent excitation. The supraoptic nucleus also receives a generalized dopaminergic input from hypothalamic sources. To determine if dopamine can influence this excitatory drive onto the magnocellular neurons, we used whole-cell patch clamp to record the effect of dopamine o...